FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2423_day_3_ul-b.35200000088a6e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2423_day_3_ul-b.35200000088a6e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211367
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA11260.532722705058027No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC9690.45844431723022044No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA8960.4239072324440428No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA7080.3349624113508731No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC4590.21715783447747283No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA3690.1745778669328703No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA3480.16464254117246307No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC3230.15281477241007346No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA3100.1466643326536309No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT3090.1461912219031353No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG3040.14382566815065737No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC2940.13909456064570155No Hit
AGACTAAACACTGCAGTTAAAGTCAGGGATTTGGGGGCACCTGGGTGGCT2910.1376752283942148No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA2840.13436345314074571No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2790.1319978993882678No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA2790.1319978993882678No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA2710.12821301338430313No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG2650.12537434888132964No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG2640.12490123813083405No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA2600.12300879512885171No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT2590.12253568437835614No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC2540.12017013062587821No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA2490.11780457687340029No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA2460.11638524462191353No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG2400.11354658011894005No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG2360.11165413711695771No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT2300.10881547261398421No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC2180.10313814360803719No Hit
AGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACC2170.10266503285754162No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG2160.10219192210704604No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC2120.1002994791050637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1550.084.193551
AGCAAAA9750.082.538472
AGCGAAA2950.071.271191
CAAAAGC11700.069.401714
GCAAAAG11700.068.782063
GCGAAAG3100.067.822582
CGAAAGC3150.066.746033
AAAAGCA12700.063.3661425
CATAGAC350.00356261462.1428579
GAGCAAA14050.059.857651
AAAGCAG15750.059.841276
GCAGGTA553.2131877E-452.7272722
AAGCAGG19450.048.4575847
GCAGGAG5250.045.571439
GAAAGCA5000.043.54
TACTATG1557.348717E-1042.0967752
CAAGGTA951.0261411E-438.1578944
TATGTTC1354.946669E-737.592595
AGCAGGA7600.036.258
ACAAGAC800.002032989236.253
Produced by FastQC (version 0.11.2)