FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2422_day_3_ul-b.35200000088953.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2422_day_3_ul-b.35200000088953.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58684
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA6301.073546452184582No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC3890.6628723331742894No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA2200.37488923727080636No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1520.2590143821143753No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA1510.2573103401267807No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA1500.2556062981391861No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC1250.2130052484493218No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG1230.20959716447413265No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1200.20448503851134892No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1040.1772203667098357No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC1010.172108240747052No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA960.16358803080907913No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA960.16358803080907913No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA950.16188398882148455No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA940.16017994683388997No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA920.15677186285870084No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA890.1516597368959171No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC880.14995569490832253No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG840.14313952695794424No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA840.14313952695794424No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC810.13802740099516053No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC760.12950719105718764No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG760.12950719105718764No Hit
GTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCGGG690.11757889714402563No Hit
AAAAAAATACAATATCTGAATTGGAAAAGCCACTGCAGGGCCTTAACAGA690.11757889714402563No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG680.11587485515643105No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT670.11417081316883648No Hit
AGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACC660.1124667711812419No Hit
AGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACA650.11076272919364734No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT650.11076272919364734No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT640.10905868720605275No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC640.10905868720605275No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG640.10905868720605275No Hit
CAATAAATATATTTAATAAAAATAGATTAACCAGGGCAACTAGGTGGCTC630.10735464521845818No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG620.1056506032308636No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC610.10394656124326904No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC610.10394656124326904No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC600.10224251925567446No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA600.10224251925567446No Hit
CAGTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGA590.10053847726807989No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCAG100.0070739733145.0145
AATCGCT100.0070739733145.05
GAGCGAA256.4138676E-6116.01
AGCAAAA4100.095.48782
TACTATG554.0745363E-1092.2727362
ATGTTCT554.0745363E-1092.2727366
ACTATGT502.2313543E-887.03
TATGTTC502.2313543E-887.05
GCAAAAG4550.086.043963
GAGCAAA4550.086.043961
CGAAAGC608.112693E-1084.5833363
CAAAAGC4750.082.421054
AGCGAAA651.5297701E-978.076931
AAAGCAG5650.075.707966
AAAAGCA5250.074.571435
ACCACTC300.001926637872.57
GCGAAAG754.7493813E-967.6666642
AAGCAGG6600.064.810617
CAATTGT702.2965287E-762.142857145
GCAGGAG1700.055.4411749
Produced by FastQC (version 0.11.2)