FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2422_day_3_ll-e.3520000008888a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2422_day_3_ll-e.3520000008888a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164722
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA27001.6391253141656852No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT22301.3537960928109178No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG20181.225094401476427No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC17711.0751447894027513No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG11560.7017884678427897No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA9170.556695523366642No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG8990.545768021272204No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT8400.5099500977404354No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA6970.4231371644346232No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA6890.41828049683709523No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA6600.4006750767960564No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA6340.3848909071040905No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG6170.3745704884593436No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA5960.36182173601583273No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC5790.3515013173710858No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT5540.336324231128811No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT5050.30657714209395226No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC4810.2920071393013684No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT4770.2895788055026044No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG4750.2883646386032224No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC4720.2865433882541494No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG4680.28411505445538543No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG4640.2816867206566214No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG4640.2816867206566214No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC4460.27075921856218355No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT4420.26833088476341954No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT4400.26711671786403757No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG4390.2665096344143466No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG4190.2543679654205267No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT4160.2525467150714537No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT4120.25011838127268976No Hit
GGATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTCTA4080.24769004747392576No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT3920.23797671227886985No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG3720.22583504328504994No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC3690.22401379293597698No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC3690.22401379293597698No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC3560.21612170808999404No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA3550.21551462464030305No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT3420.2076225397943201No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT3400.20640837289493813No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT3360.20398003909617418No Hit
CAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGA3350.2033729556464832No Hit
CAAACAGCAGACAAGAGAATAATGGACATGATTCCAGAGACACATCAGGA3300.2003375383980282No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA3170.19244545355204526No Hit
GCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGG3100.18819586940420832No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT3090.1875887859545173No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC3020.18333920180668034No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTC3010.18273211835698935No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG2990.18151795145760738No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG2940.1784825342091524No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC2920.1772683673097704No Hit
GCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGAT2880.17484003351100644No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG2870.17423295006131545No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA2840.17241169971224246No Hit
GTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTTTGATTATGGCC2820.17119753281286046No Hit
CATCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT2750.1669479486650235No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC2690.16330544796687751No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2660.16148419761780455No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGC2630.15966294726873156No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT2590.1572346134699676No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA2580.15662753002027657No Hit
GGGCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT2490.15116377897305763No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCCTTT2470.14994961207367563No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTC2400.14570002792583867No Hit
AGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTG2310.14023627687861973No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT2300.13962919342892874No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC2280.13841502652954674No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT2280.13841502652954674No Hit
ATGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGA2270.13780794307985575No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG2260.13720085963016476No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCCTTTTC2260.13720085963016476No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC2250.13659377618047375No Hit
GAGAAGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG2230.13537960928109177No Hit
GTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTAGTGAGTATCT2200.13355835893201878No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC2190.1329512754823278No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA2170.1317371085829458No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA2130.12930877478418182No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG2100.12748752443510886No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2100.12748752443510886No Hit
CTCCTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG2030.12323794028727189No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA1970.11959543958912591No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTT1930.11716710579036194No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTC1920.11656002234067095No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA1910.11595293889097996No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG1910.11595293889097996No Hit
GGATGATGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGA1880.11413168854190697No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA1850.11231043819283398No Hit
GATTTCCATTTCATCGACGAACGGGGTGAATCAATAATTGTAGAATCTGG1840.111703354743143No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA1840.111703354743143No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG1810.10988210439407001No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC1810.10988210439407001No Hit
GATTACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAATTGCTTCTCATT1800.10927502094437902No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC1790.10866793749468803No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA1770.10745377059530603No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT1750.10623960369592403No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA1740.10563252024623304No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT1740.10563252024623304No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG1740.10563252024623304No Hit
GGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATT1720.10441835334685107No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG1690.10259710299777808No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT1680.10199001954808709No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA1680.10199001954808709No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGGG253.6974598E-8145.0145
TATATAA100.0070855245144.999982
TCGATGG100.0070855245144.999983
TCCGTGG100.0070855245144.999982
TATAAAT100.0070855245144.999984
GAGCGAA1800.0140.972231
GAGCAAA9700.0129.304121
CACGGAA203.8602634E-4108.749993
AAGGTAC203.8602634E-4108.749999
GCAGGTA3400.098.0882349
AGCAAAA13350.093.951312
AGCAGGT9350.089.171128
CAAAAGC13950.088.870974
GACAGTA502.2568202E-887.07
AAGCAGG17300.086.748567
AAAAGCA14400.086.597235
AGCAGGC4000.083.3758
AAAGCAG18250.082.232876
CAAGGTA451.2283017E-680.555568
GCAGGTC6250.080.039999
Produced by FastQC (version 0.11.2)