FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2419_day_1_ul-b.352000000887f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2419_day_1_ul-b.352000000887f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137241
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA12510.9115351826349269No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC10550.7687207175698224No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA5160.3759809386407852No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA4940.35995074358245716No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA3670.2674127993821088No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA3390.24701073294423678No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT3130.22806595696621274No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA2900.21130711667796068No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC2870.2091211809881887No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA2570.1872618240904686No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA2390.17414620995183655No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA2380.17341756472191255No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC2290.16685975765259653No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA2270.16540246719274854No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC2210.1610305958132045No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA2150.1566587244336605No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA2120.15447278874388848No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA2070.1508295625942685No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG2050.14937227213442048No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC2030.14791498167457245No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG2010.14645769121472446No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC2000.14572904598480046No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG1970.14354311029502848No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1940.14135717460525646No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC1920.13989988414540844No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG1850.13479936753594043No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT1710.1245983343170044No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT1650.12022646293746038No Hit
GGTTAATCTATTTTTATTAAATATATTTATTGTCATGGTTGGGCTTGAGT1610.11731188201776438No Hit
CAGTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGA1610.11731188201776438No Hit
CAATAAATATATTTAATAAAAATAGATTAACCAGGGCAACTAGGTGGCTC1590.11585459155791637No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA1590.11585459155791637No Hit
AGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACA1540.11221136540829635No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC1520.11075407494844836No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT1490.10856813925867635No Hit
GGGTAAATATAAAAAAACGACTCTGATACATATCACACTTGAATTGCTGA1450.10565355833898034No Hit
CTGATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCA1450.10565355833898034No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG1450.10565355833898034No Hit
CTTAACCACTCAGACACCCAGGCATCCCAACATTAAACAGATTTTTACCT1440.10492491310905633No Hit
AGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACC1410.10273897741928431No Hit
GTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCGGG1410.10273897741928431No Hit
TCCCAGGACTTTGGAATCATGACCTGAGCTGAAGGCAGACACTTAACAAC1400.10201033218936031No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC1380.10055304172951231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1550.0102.903221
AGCAAAA7600.085.855262
GCAAAAG7550.085.463583
CAAAAGC7700.083.7987064
AGCGAAA2100.075.952382
AAAGCAG11100.072.56
GAGCAAA9600.069.4791641
AAAAGCA9350.069.01075
GCGAAAG2350.067.8723453
CGAAAGC2500.063.84
CAAGGTA502.004109E-458.04
AAGCAGG14250.057.4912267
GCAGGAG3800.057.2368439
GAAAGCA3250.049.0769235
GATACCA604.9300765E-448.3333321
AGCAGGA4950.048.3333328
ATACCAC604.9300765E-448.3333322
GCAGGGG3750.042.533339
TACCACC700.001053453541.428573
AGCAGGG10900.040.5733958
Produced by FastQC (version 0.11.2)