FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2417_day_1_ll-e.35200000088a01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2417_day_1_ll-e.35200000088a01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157483
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG16071.0204276017093907No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC5960.37845354736701736No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA5860.3721036556326715No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA5700.361943828857718No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC5590.35495894794993743No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC5240.3327343268797267No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA5070.32193951093133866No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT4680.2971749331673895No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT4380.27812525796435167No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA4360.2768552796174825No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT4310.2736803337503096No Hit
GAGCAAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAA4270.2711403770565712No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC4260.2705053878831366No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG4030.25590063689414094No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG3890.24701078846605667No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT3880.24637579929262204No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT3840.2438358425988837No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG3790.24066089673171073No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC3430.21780128648806538No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT3360.21335636227402324No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT3310.21018141640685029No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG3280.2082764488865465No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT3250.20637148136624267No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC3140.19938660045846218No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG3130.1987516112850276No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG3090.1962116545912892No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA3070.19494167624442002No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC3000.19049675203037789No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA2830.1797019360819898No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA2670.1695421093070363No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACG2640.16763714178673256No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA2510.15938228253208284No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG2490.15811230418521363No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC2480.15747731501177903No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG2410.1530323907977369No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG2390.15176241245086772No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA2380.15112742327743311No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG2380.15112742327743311No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT2330.14795247741026016No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT2270.1441425423696526No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC2230.14160258567591422No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT2140.1358876831150029No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT2140.1358876831150029No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT2140.1358876831150029No Hit
GTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGA2140.1358876831150029No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA2120.1346177047681337No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC2110.1339827155946991No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC2090.13271273724782992No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT2070.13144275890096074No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC2070.13144275890096074No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT2010.12763282386035318No Hit
GGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGAGATTAC2010.12763282386035318No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT2000.12699783468691858No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC1990.12636284551348398No Hit
TTTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTT1980.1257278563400494No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTC1970.12509286716661483No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA1960.12445787799318021No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT1940.12318789964631102No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG1920.12191792129944184No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA1910.12128293212600724No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG1910.12128293212600724No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT1870.11874297543226887No Hit
TTCAAGATCCATTCCACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGAT1840.11683800791196511No Hit
TACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTG1830.1162030187385305No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG1830.1162030187385305No Hit
GTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTT1810.11493304039166133No Hit
TAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTT1800.11429805121822674No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAG1780.11302807287135754No Hit
ATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTC1760.11175809452448836No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC1760.11175809452448836No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC1760.11175809452448836No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT1760.11175809452448836No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCA1740.11048811617761917No Hit
ACCTTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTC1740.11048811617761917No Hit
ACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGA1710.10858314865731539No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTCATG1700.1079481594838808No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTA1700.1079481594838808No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT1700.1079481594838808No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1690.1073131703104462No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT1690.1073131703104462No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT1680.10667818113701162No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT1680.10667818113701162No Hit
TTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGAC1680.10667818113701162No Hit
ATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTC1680.10667818113701162No Hit
TTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTGT1670.10604319196357702No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAG1640.10413822444327325No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC1630.10350323526983865No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA1610.10223325692296946No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA1590.10096327857610028No Hit
GTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA1590.10096327857610028No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGCG100.007085229145.000029
CGGCGGG151.2280532E-4145.0145
GAGCGAA1300.0128.269231
GAGCAAA5600.0104.866071
GCAGGGT3700.084.256759
AAGCAGG8950.084.245817
CTAGTGC353.4282708E-582.857149
CTAATAA353.4282708E-582.857149
AGCAGGC900.080.555568
GGTCGTT651.553417E-978.076927
AGGTCGT651.553417E-978.076926
AAGGTCG755.4569682E-1177.333335
GCAGGTC1650.074.6969769
AGCAGGT3800.074.407898
GCGAAAG2250.074.111113
AGCGAAA2250.074.111112
AGCAGGG5650.073.14168
ATTAGTA300.001932703972.51
CGAAAGC2300.072.54
CCAGCCC300.001932703972.58
Produced by FastQC (version 0.11.2)