FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2416_day_1_nw.35200000088662.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2416_day_1_nw.35200000088662.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210400
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA7670.36454372623574144No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG6550.31131178707224333No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG6160.29277566539923955No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT3930.186787072243346No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG3350.15922053231939162No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT3260.15494296577946767No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2980.14163498098859317No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT2830.13450570342205323No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA2770.13165399239543726No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA2510.11929657794676805No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT2450.11644486692015207No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT2400.11406844106463879No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA2290.10884030418250949No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT2290.10884030418250949No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC2280.10836501901140684No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT2240.1064638783269962No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT2220.10551330798479086No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2190.10408745247148289No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG2170.10313688212927757No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG2160.10266159695817491No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC2150.10218631178707226No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT2120.10076045627376426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1000.0137.751
TTATAGG352.7541137E-7103.571431
GCAGGTA2100.0100.119069
GTATAGC259.376375E-487.01
AGCGAAA1600.086.093752
GCAGGGT2550.085.294119
GCGAAAG1800.076.527783
CGAAAGC1850.074.4594654
GCAAAAG7200.062.4305573
GTAGAAA1051.6370905E-1162.142861
ACCGTCT350.003562591762.1428578
CACCGTC350.003562591762.1428577
CGATCCC350.003562591762.1428575
CTAAGGA1350.059.074073
GTGTTTT502.0065813E-458.09
AGCAGGG4850.056.8041238
AGTAGAA902.0390871E-856.388891
GTCTAAG1301.8189894E-1255.7692341
TCTAAGG1301.8189894E-1255.7692342
ACTCAGC400.006044273354.3751
Produced by FastQC (version 0.11.2)