FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2186_day_3_nw.35200000088649.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2186_day_3_nw.35200000088649.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117772
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG5380.4568148626159019No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA5060.4296437183710899No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA4040.34303569609075163No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT3410.28954250585877794No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG3120.2649186563869171No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2850.24199300343035698No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT2310.1961416975172367No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG2190.1859525184254322No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2010.17066874978772542No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA1580.1341575247087593No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1530.12991203342050742No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC1510.12821383690520666No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG1500.1273647386475563No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA1500.1273647386475563No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT1480.12566654213225556No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT1470.12481744387460518No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT1430.12142105084400366No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT1430.12142105084400366No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1410.11972285432870292No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC1360.11547736304045104No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT1360.11547736304045104No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT1340.1137791665251503No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT1240.10528818394864654No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG1240.10528818394864654No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA1210.10274088917569542No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC1190.10104269266039466No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1180.10019359440274428No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGG100.007082976145.03
CACGGGT100.007082976145.04
CCCTACA151.2272724E-4145.02
GAGCGAA400.0145.01
CGCCGTT100.007082976145.0145
CCTACAC203.8574985E-4108.753
CACCTAC301.5935766E-596.6666643
CCACCTA301.5935766E-596.6666642
AGCGAAA1200.090.6251
CGAAAGC1250.087.000013
GCGAAAG1300.083.653852
CCCCTAC353.4246717E-582.857141
CCTACTA353.4246717E-582.857145
GCAGGGT2350.080.212779
GAGCAAA5200.079.471151
GTAGAAA1100.072.51
ACACTGG406.638821E-572.56
AGTAGAA607.970266E-872.51
GCAGGTA1350.069.814819
AGCAGGG3650.067.534258
Produced by FastQC (version 0.11.2)