FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2182_day_1_nw.3520000008863c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2182_day_1_nw.3520000008863c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149126
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA6180.41441465606265837No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG5220.3500395638587503No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG4650.31181685286267985No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG2830.1897724072261041No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT2660.17837265131499538No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT2530.16965519091238282No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2030.13612649705618068No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT1950.13076190603918833No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT1930.12942075828494026No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA1860.12472674114507194No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC1850.1240561672679479No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA1840.12338559339082386No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT1830.12271501951369984No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT1790.12003272400520365No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA1730.11600928074245939No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT1620.10863296809409492No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC1570.10528009870847471No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG1560.10460952483135066No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT1540.1032683770771026No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTAC151.2279235E-4145.03
TTGTGTA100.0070848563144.999984
GAGCGAA650.0133.846151
AGTAGAA501.8189894E-12116.01
CCTACTA203.859538E-4108.749995
CCACCTA203.859538E-4108.749992
TTATAGG352.7501483E-7103.571421
CAATTCG406.0918137E-790.624999
GTAGTTT259.370978E-487.08
CAAGGTA353.4276738E-582.857144
TCTAGCT451.2277287E-680.555562
GCAGGTA1550.074.838719
GCAGGGT2650.073.867939
GACAGTA300.001932502172.57
CGAAAGC1200.072.53
GCTATAC300.001932502172.53
TCCACCT300.001932502172.51
AGCGAAA1200.072.52
GGTAGTT300.001932502172.57
GCAAAAG7000.065.253
Produced by FastQC (version 0.11.2)