FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2181_day_1_ul-b.35200000088a52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2181_day_1_ul-b.35200000088a52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197364
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA14590.7392432257149226No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC10980.5563324618471454No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA8960.4539835025637907No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA7100.35974139154050383No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC5020.25435236415962387No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA4490.22749842929814962No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA4030.204191240550455No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA4020.20368456253420075No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA3810.19304432419286194No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT3570.18088405180276038No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA3240.16416367726637077No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC3220.1631503212338623No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC3140.15909689710382846No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG3070.15555015099004885No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA2980.15099004884376077No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC2930.14845665876248962No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA2910.14744330272998116No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA2870.1454165906649642No Hit
AGACTAAACACTGCAGTTAAAGTCAGGGATTTGGGGGCACCTGGGTGGCT2830.1433898785999473No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA2760.1398431324861677No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG2710.13730974240489655No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA2670.1352830303398796No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG2610.13224296224235424No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT2510.12717618207981193No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG2500.1266695040635577No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA2480.12565614803104924No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC2430.12312275794977807No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC2390.12109604588476114No Hit
GGTTAATCTATTTTTATTAAATATATTTATTGTCATGGTTGGGCTTGAGT2380.12058936786850692No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC2350.11906933381974423No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT2210.11197584159218499No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT2200.11146916357593077No Hit
CAATAAATATATTTAATAAAAATAGATTAACCAGGGCAACTAGGTGGCTC2170.10994912952716808No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC2150.10893577349465962No Hit
CTGTTAAGGCCCTGCAGTGGCTTTTCCAATTCAGATATTGTATTTTTTTA2140.10842909547840537No Hit
TCCCAGGACTTTGGAATCATGACCTGAGCTGAAGGCAGACACTTAACAAC2100.10640238341338847No Hit
GTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCGGG2040.10336231531586307No Hit
CTGATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCA2000.10133560325084616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAAAA10050.092.33832
GCAAAAG10850.085.529953
GAGCGAA1900.083.947371
CAAAAGC12050.077.6141054
AAAAGCA13000.071.942315
AAAGCAG15500.070.629036
GAGCAAA14350.065.679441
GCAGGAG5100.061.127459
AGCGAAA2750.058.01
CGAAAGC3000.053.1666643
GCGAAAG3000.053.1666642
AAGCAGG21750.051.6666687
AGCAGGA7900.044.0506328
CATACCG350.003658696841.428575
ATACCGA350.003658696841.428576
GAAAGCA4200.037.976194
GCAGGGG5850.035.940179
GTAGAAA2650.035.566041
AGCAGGG17700.033.587578
TAAAGGG900.003621232732.222224
Produced by FastQC (version 0.11.2)