FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2181_day_1_nw.352000000886cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2181_day_1_nw.352000000886cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96711
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA4540.46943987757338873No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG4030.4167054419869508No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG3910.40429733949602426No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT2060.21300575942757288No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT1780.18405352028207753No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG1580.16337334946386659No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT1440.14889722989111892No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA1420.1468292128092978No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1310.1354551188592818No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA1300.13442111031837123No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT1270.1313190846956396No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT1230.12718305053199738No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT1170.1209789992865341No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT1140.11787697366380245No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA1130.11684296512289193No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG1090.11270693095924973No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA1080.11167292241833918No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT1050.10857089679560752No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1040.107536888254697No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT1030.10650287971378644No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC1000.1034008540910548No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC1000.1034008540910548No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC980.10133283700923369No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA980.10133283700923369No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA980.10133283700923369No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT970.10029882846832314No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACTT100.0070810313145.05
GCTATAC100.0070810313145.03
ATAGATA100.0070810313145.02
TAGATAC100.0070810313145.03
GATAGAT100.0070810313145.01
GAGCGAA501.8189894E-12116.01
GCAGGTA1050.0103.571439
GATGGGG259.360942E-487.01
TATGTGA259.360942E-487.08
GCAAAAG4300.082.616283
GCAGGGT1600.081.56259
AGCGAAA651.544322E-978.076922
CGAAAGC702.7775968E-972.54
GCGAAAG702.7775968E-972.53
AAGCAGG5700.071.228077
AAAGCAG5700.071.228076
CAAAAGC5200.068.317314
GAGCAAA5150.066.1650541
GAGACCC554.033949E-665.909094
GACGACT554.033949E-665.909097
Produced by FastQC (version 0.11.2)