FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2181_day_1_ll-e.352000000887a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2181_day_1_ll-e.352000000887a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73918
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA6620.8955870018128196No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC4350.5884899483211126No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA2170.293568548932601No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA1690.22863172704889204No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA1650.22322032522524962No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1290.17451770881246786No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA1280.17316485835655726No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC1250.16910630698882545No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1250.16910630698882545No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1240.16775345653291485No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1130.1528721015178982No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT1100.1488135501501664No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1040.14069644741470277No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1030.13934359695879217No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC930.12581509239968613No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC920.12446224194377553No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG910.12310939148786493No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA880.11905084012013312No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT880.11905084012013312No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT870.11769798966422251No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC830.1122865878405801No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA830.1122865878405801No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC820.11093373738466951No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA810.10958088692875889No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC760.10281663464920589No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGGC100.0070776753144.999983
GAGCGAA1000.0123.251
TCGCTCC203.8517502E-4108.749995
CATACCG203.8517502E-4108.749995
GCTTTCG203.8517502E-4108.749991
CGCTCCC259.352138E-487.06
CAGCTCG259.352138E-487.09
CTTTCGC259.352138E-487.02
ATACCGA259.352138E-487.06
AGCAAAA3950.080.759492
GCGAAAG1550.079.516133
AGCGAAA1550.079.516132
GCAAAAG4100.077.804883
CGAAAGC1600.077.031244
CAAAAGC4300.074.186044
AAAGCAG6100.072.56
GAGCAAA4600.069.347831
TTTCGCT350.0035534562.142863
TATACAG350.0035534562.142865
ATACAGC350.0035534562.142866
Produced by FastQC (version 0.11.2)