FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2020_day_14_ul-b.352000000889d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2020_day_14_ul-b.352000000889d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114808
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA4070.3545049125496481No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC4000.3484077764615706No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT2850.24824054072886906No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA2560.2229809769354052No Hit
GAGCAAAAGCAGGGACTTTTGGGCAGCATTAAGATGTATGAGGTGTATTC2470.21514180196501986No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG2230.19423733537732563No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA2220.1933663159361717No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC2170.18901121873040205No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2140.1863981604069403No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA2090.18204306320117064No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC2070.1803010243188628No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1910.16636471326039998No Hit
GAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAAT1790.15591247996655286No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC1710.14894432443732145No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA1470.1280398578496272No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC1460.12716883840847326No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT1440.12542679952616542No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG1410.12281374120270364No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1410.12281374120270364No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT1400.12194272176154972No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT1390.1210717023203958No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT1380.12020068287924186No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG1370.11932966343808793No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG1350.11758762455578009No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC1340.11671660511462616No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1320.1149745662323183No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG1290.11236150790885652No Hit
GTAGAAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCC1260.10974844958539474No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1190.10365131349731727No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT151.2271927E-4145.04
GTACGAG151.2271927E-4145.03
CGCAAAA100.0070827473144.999981
GAGCGAA1350.0102.037031
AGCAAAA7100.099.049292
GAGCAAA7550.093.145691
GCAAAAG7750.090.7419363
ATGTACG259.365444E-487.01
CAAAAGC8200.085.762194
AAAAGCA8500.081.882365
AAAGCAG10950.075.4794546
TGTACGA300.001931364972.52
AGCGAAA1950.070.641032
GCAGGAT1050.069.0476159
TCCTGTA350.003558469562.1428532
GCGAAAG2250.061.2222253
GCAGGTT753.7317477E-758.09
CGAAAGC2400.054.3754
CAACCCT400.006037305654.374996145
GGTATTC953.106834E-853.421055145
Produced by FastQC (version 0.11.2)