FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2020_day_14_ll-e.352000000887b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2020_day_14_ll-e.352000000887b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101704
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA6730.6617242193030756No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC4800.47195783843309996No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA2320.22811295524266498No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2090.2054983088177456No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA2040.2005820813340675No Hit
GAGCAAAAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCA1720.16911822543852748No Hit
CTTAACCACTCAGACACCCAGGCATCCCAACATTAAACAGATTTTTACCT1680.16518524345158497No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1660.16321875245811374No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG1650.16223550696137812No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA1630.16026901596790688No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1440.14158735152993No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1360.13372138755604498No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC1350.13273814205930937No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1240.12192244159521749No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1220.11995595060174626No Hit
GAGCAAAAGCAGGCAGAGTGGGAGAGGGAGAAGCAGGCTCCCGCTGAGCA1200.11798945960827499No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1140.11208998662786125No Hit
GTAGAAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCC1070.10520726815071188No Hit
TCCCAGGACCCTGCGATCATGAACTGAGTCAAAGGCAGACACTTAACCAC1050.10324077715724062No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1050.10324077715724062No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG1030.10127428616376938No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1030.10127428616376938No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA203.8559697E-4108.759
GAGCGAA1100.0105.454551
AGCAAAA6600.095.5681842
CAAAAGC7250.088.04
GCAAAAG7300.086.404113
GAGCAAA7750.082.3225861
AGCGAAA1450.075.02
AAAAGCA8700.073.333335
AAAGCAG10600.070.448116
GTAGAAA1250.069.6000061
CGAAAGC1700.063.970594
GCGAAAG1800.060.4166683
AGCAGGT1007.075869E-1058.08
AAGCAGG12900.057.325587
ACAAGGC400.006035330754.3753
GCAGGAG2750.052.7272729
GCAGGGG2900.050.09
GTATACT450.00961444848.3333324
AGCAGGA3400.046.9117668
GAAAGCA2550.045.4901965
Produced by FastQC (version 0.11.2)