FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2017_day_14_ul-b.35200000088860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2017_day_14_ul-b.35200000088860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139794
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA9410.6731333247492739No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC7570.5415110805900111No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA5790.41418086613159366No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA4940.35337711203628197No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA4210.3011574173426613No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA3040.2174628381761735No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC2800.20029471937279142No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA2780.19886404280584288No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT2420.1731118646007697No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT2420.1731118646007697No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA2400.1716811880338212No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA2400.1716811880338212No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG2350.1681044966164499No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA2140.15308239266349058No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA2130.1523670543800163No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT2090.14950570124611928No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT1970.14092164184442824No Hit
GAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAAT1950.13949096527747973No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC1940.13877562699400547No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG1930.1380602887105312No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA1850.1323375824427372No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC1840.1316222441592629No Hit
GGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAG1770.12661487617494313No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT1770.12661487617494313No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC1730.1237535230410461No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG1710.1223228464740976No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA1710.1223228464740976No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1700.12160750819062334No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA1640.11731547848977782No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG1620.11588480192282931No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1610.11516946363935504No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC1600.11445412535588079No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1580.11302344878893228No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT1580.11302344878893228No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT1570.11230811050545803No Hit
GAGCGAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA1550.11087743393850952No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1510.1080160808046125No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG1480.10587006595418973No Hit
GCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACT1460.10443938938724123No Hit
CCCCACTCAGCAGGGAGCCCAATGTGGGACTCGATCCCAGGACCCTGAGA1450.10372405110376696No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTG100.007084382145.000027
GAGCGAA1650.0118.636361
AGCAAAA8850.093.389832
GCAAAAG9650.085.6476753
GAGCAAA10250.080.6341551
AGCGAAA2450.079.897962
AAAAGCA10700.077.920565
GGTACCA406.643164E-572.500011
CAAAAGC11400.072.500014
GCGAAAG2700.072.499993
AAAGCAG14550.070.756016
CGAAAGC2800.069.910714
GCAGGAT1650.065.909099
GCAGGGG5250.056.6190459
GAAAGCA3600.054.3755
GCAGGTT1205.820766E-1154.3759
AGCAGGA5100.052.5980388
AAGCAGG20050.050.6234367
GCAGGAG3250.049.0769239
ACAAGGT951.908964E-645.7894748
Produced by FastQC (version 0.11.2)