FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2017_day_14_ll-e.35200000088acb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2017_day_14_ll-e.35200000088acb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133742
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA4890.36562934605434344No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC4550.34020726473359153No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA4080.30506497584902276No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA3120.23328498153160562No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA2540.18991790163149944No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA2440.18244081889010183No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG2390.17870227751940304No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT2330.17421602787456447No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT2220.16599123685902709No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA2220.16599123685902709No Hit
ATATTATCTTACCCCTCTGCCATAGGCCTTTATTGTTTAGGTAATTAAAA2180.16300040376246802No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1910.14281228036069446No Hit
GATGTATGTAGTATGATTCCACTTATATAAAAACAAACAAAAAAAGGAAA1840.13757832244171614No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC1770.13234436452273782No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT1750.13084894797445829No Hit
ATGTAGTATGATTCCACTTATATAAAAACAAACAAAAAAAGGAAAAGGTC1670.1248672817813402No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1650.12337186523306067No Hit
GAGCAAAAGCAGGGTGTGGGGAGGCATTTTATTAAAAAAAATTAACAAAC1630.12187644868478115No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1610.12038103213650163No Hit
GTATTACTTAAGCAAAAGTGTTCTCTTGGATAACCAATGACCAGGCGATT1600.11963332386236186No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT1530.11439936594338353No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1500.11215624112096426No Hit
ATTAATATCTATATTATCTTACCCCTCTGCCATAGGCCTTTATTGTTTAG1460.10916540802440519No Hit
ATATAAAAGAATATACATGATTTTATTTTTGGCTCAATCAAGTTGCAGAC1440.10766999147612567No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG1410.1054268666537064No Hit
GGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAG1400.10467915837956664No Hit
CTATATTATCTTACCCCTCTGCCATAGGCCTTTATTGTTTAGGTAATTAA1400.10467915837956664No Hit
GTAATATACCTCCAAAGCTTGAATTTTAAAGCTCTATCCTCGAAGATTAA1390.10393145010542687No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG1380.10318374183128709No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1150.0107.1739041
GAGCAAA8200.080.457311
CAAGGTA554.3652108E-879.090914
AGCAAAA8000.078.843752
AAAAGCA8350.075.5389255
GCAAAAG8600.073.3430253
CAAAAGC9050.069.696134
AGCGAAA2000.068.8751
AAAGCAG11000.068.5454566
GCGAAAG2100.065.595242
GCAGGGG4550.065.329679
GTCCCGG451.19009346E-464.444441
CGAAAGC2250.061.2222183
GTCATAC400.006039472854.3751
CCTTATA805.835118E-754.3752
AAGCAGG15900.047.877367
GTAGAAA2000.047.1252
GAAAGCA3000.045.9166684
GCAGGAG2250.045.1111079
GCAGGAT1453.8016879E-1045.0000049
Produced by FastQC (version 0.11.2)