FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2016_day_8_nw.35200000088513.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2016_day_8_nw.35200000088513.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106637
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG4790.44918743025403934No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG4640.43512101803314046No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA4090.3835441732231777No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC3810.357286870410833No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA2900.27195063627071275No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2540.23819124694055535No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG2320.21756050901657023No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2290.21474722657239043No Hit
GCGTGAACACAAATCCTAAAATTCCCTTAGTCAGAGGTGACAAGATTGGT1620.15191725198570852No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC1500.14066412220898938No Hit
GGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGC1390.13034875324699682No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1170.1097180153230117No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1090.10221592880519895No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG1070.10034040717574574No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG100.007082041145.09
CTATCTA100.007082041145.04
GAGCAAA4650.096.666671
AGCAAAA5450.082.477062
GAGCGAA1350.080.555561
AGCGAAA1550.074.838712
GCGAAAG1500.072.53
ATGGACG300.001930985172.54
CGAAAGC1550.070.1612854
GCAAAAG6450.066.317833
CTGACGG451.1889902E-464.44444145
ATAATCG350.00355777162.1428575
ATATATC350.00355777162.1428572
CAAGGTA350.00355777162.1428574
CAGGCTA350.00355777162.1428579
ATCGATG350.00355777162.1428578
AATCGAT350.00355777162.1428577
TAATCGA350.00355777162.1428576
CAAAAGC7150.060.8391574
AAAGCAG9350.058.9304856
Produced by FastQC (version 0.11.2)