FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2015_day_8_ul-b.352000000888e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2015_day_8_ul-b.352000000888e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106446
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA9280.8718035435807828No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC7830.7355842398962854No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA4960.4659639629483494No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA4520.42462845010615713No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA2900.2724386073689946No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2600.24425530315840896No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA2530.23767919884260563No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA2500.23486086842154705No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC2470.23204253800048852No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA2350.22076921631625424No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT2280.21419311200045094No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA2210.20761700768464764No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC1960.18413092084249288No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1930.18131259042143436No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA1850.17379704263194484No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA1830.17191815568457247No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT1830.17191815568457247No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1800.1690998252635139No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC1780.16722093831614152No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG1780.16722093831614152No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA1770.16628149484245533No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC1760.16534205136876914No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT1750.16440260789508296No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC1680.15782650357927963No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA1500.14091652105292823No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG1500.14091652105292823No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1500.14091652105292823No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1470.13809819063186968No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG1440.13527986021081112No Hit
GGTTAATCTATTTTTATTAAATATATTTATTGTCATGGTTGGGCTTGAGT1430.13434041673712493No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG1400.13152208631606635No Hit
CAATAAATATATTTAATAAAAATAGATTAACCAGGGCAACTAGGTGGCTC1320.12400653852657685No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT1320.12400653852657685No Hit
AGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACC1280.12024876463183211No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT1250.11743043421077352No Hit
CTGATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCA1250.11743043421077352No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC1240.11649099073708735No Hit
GGGTAAATATAAAAAAACGACTCTGATACATATCACACTTGAATTGCTGA1220.11461210378971498No Hit
CAGTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGA1220.11461210378971498No Hit
TTTTTATATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGA1220.11461210378971498No Hit
ATATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGT1210.11367266031602878No Hit
ATTCAAGTGTGATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTG1190.1117937733686564No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT1180.11085432989497022No Hit
AGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACA1170.10991488642128402No Hit
GAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAAAGAACT1160.10897544294759785No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC1100.10333878210548073No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT1080.10145989515810833No Hit
GGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAG1080.10145989515810833No Hit
ATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCCAC1080.10145989515810833No Hit
CTTTAAAACACTTATTATGAATAAGGATAAGAATATAAAAAATACGACCA1070.10052045168442215No Hit
TCCCAGGACCCTGGGATCATGACCTGAGGTGAAGGCAGACAATCGACTGA1070.10052045168442215No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTA100.0070820223145.02
GTTGGCG100.0070820223145.08
GAGCGAA950.0106.8420941
AGCAAAA6900.0101.920292
GCAAAAG8150.087.177923
AGCGAAG259.363544E-487.02
GAGCAAA8300.086.47591
AAAAGCA9500.073.263165
AAAGCAG10850.072.834116
GTTCGAG300.001930974972.5145
AGCGAAA1500.072.51
TGCACAG406.635879E-572.57
CAAAAGC9750.072.1282044
GTGTAAG350.003557752462.1428571
ACAAGGC350.003557752462.1428578
CGAAAGC1800.060.4166683
GCGAAAG1850.058.7837872
GCAGGAT753.7291284E-758.0000049
GTGCACA502.0020534E-458.06
TGTGCAC502.0020534E-458.05
Produced by FastQC (version 0.11.2)