FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2006_day_3_nw.352000000885f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2006_day_3_nw.352000000885f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147914
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA4860.3285693037846316No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG4660.31504793325851504No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG4240.2866530551536704No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA3580.24203253241748585No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT3110.21025731168111203No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA2240.1514393498925051No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2040.13791797936638858No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1970.1331854996822478No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT1810.12236840326135456No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG1810.12236840326135456No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA1720.11628378652460214No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA1710.11560771799829632No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC1690.11425558094568465No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT1660.11222737536676716No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT1600.10817096420893221No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA1590.10749489568262639No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG1570.10614275863001472No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA1540.10411455305109726No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC1510.10208634747217978No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG1500.10141027894587396No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG1500.10141027894587396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA550.0145.01
GCAGGTA1250.081.29
GAGCAAA6500.080.307691
CGAAAGC1400.077.678573
AGCGAAA1400.077.678571
GCAGGGT2500.075.49
GCGAAAG1450.075.02
TAGCAAA300.001932471472.58
GCAAAAG7550.068.178813
AGCAAAA8200.063.6585352
AAGCAGG9550.062.2513127
GGATAGC350.003560499362.1428575
AGTAGAA954.5110937E-1061.0526351
CAAAAGC8650.060.346824
AAAGCAG10150.059.2857176
AGCAGGG4800.057.3958328
GTAGAAA1153.8198777E-1156.7391321
CAATTCG400.00604073754.3759
AAAAGCA9950.053.1909565
CATACTC901.3158715E-648.3333323
Produced by FastQC (version 0.11.2)