FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2005_day_3_ll-e.35200000088abe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2005_day_3_ll-e.35200000088abe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220286
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA35901.6296995723740955No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT25391.1525925387904816No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC23251.0554461018857304No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20500.9306083909099988No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA16960.7699082102357844No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG12610.572437649237809No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA12370.5615427217344725No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC11910.5406607773530774No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG11600.5265881626612676No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11470.5206867435969603No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT10800.49027173765014576No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG10730.4870940504616726No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT10010.4544092679516628No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA9360.42490217263012625No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT9330.42354030669220927No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC9210.41809284294054094No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT9200.41763888762790197No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG9180.41673097700262385No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG9120.4140072451267897No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA8670.3935792560580336No Hit
GGCTATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACT8180.37133544573872146No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT8150.3699735798008044No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT7490.34001252916662883No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC6800.3086896125945362No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC6680.3032421488428679No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT6590.2991565510291167No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG6390.2900774447763362No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG6360.28871557883841914No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA6270.2846299810246679No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT6030.2737350535213314No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC6010.2728271428960533No Hit
GCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCA5990.27191923227077525No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA5910.2682875897696631No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG5670.2573926622663265No Hit
GGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAA5660.2569387069536875No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG5640.2560307963284094No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC5490.24922146663882405No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG5470.24831355601354602No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT5160.23424094132173628No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC5130.23287907538381922No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG5000.22697765631951192No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG4830.21926041600464852No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC4780.21699063944145336No Hit
GAGCAAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAA4660.2115431756897851No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA4430.20110220349908756No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT4420.20064824818644852No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC4400.1997403375611705No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA4330.19656265037269732No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC4300.19520078443478023No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA4260.19338496318422413No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT4240.1924770525589461No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA4040.18339794630616563No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG4020.1824900356808876No Hit
GTATCCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGA3950.1793123484924144No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG3870.17568070599130223No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG3810.17295697411546807No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA3700.1679634656764388No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT3690.1675095103637998No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA3570.1620620466121315No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG3570.1620620466121315No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG3570.1620620466121315No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT3570.1620620466121315No Hit
GTTCATTGATGCTTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGT3520.1597922700489364No Hit
CCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCAT3470.15752249348574127No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA3420.15525271692254616No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTC3380.15343689567199004No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG3340.15162107442143394No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC3320.1507131637961559No Hit
GGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATT3310.1502592084835169No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA3290.14935129785823884No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG3280.1488973425455998No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3270.1484433872329608No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT3250.14753547660768274No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG3250.14753547660768274No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG3230.1466275659824047No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA3200.1452657000444876No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC3170.14390383410657057No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA3160.14344987879393153No Hit
CCCAATAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTA3130.14208801285601447No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA2970.13482472785379007No Hit
GCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTG2930.13300890660323397No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC2930.13300890660323397No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC2880.13073913004003887No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT2860.1298312194147608No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA2860.1298312194147608No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA2840.12892330878948277No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTT2820.1280153981642047No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2810.1275614428515657No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA2740.12438375566309252No Hit
GCTTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTAT2730.1239298003504535No Hit
CTATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCT2640.11984420253670228No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTT2550.11575860472295109No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2550.11575860472295109No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT2530.11485069409767303No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT2490.11303487284711693No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC2460.11167300690919986No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT2460.11167300690919986No Hit
GTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGG2410.10940323034600474No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA2290.10395576659433645No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA2280.10350181128169743No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA2260.10259390065641939No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA2250.10213994534378036No Hit
GGATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTC2230.10123203471850231No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC2220.10077807940586328No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC100.0070871348145.04
TTAAGGT100.0070871348145.0145
AACGCAG100.0070871348145.06
GGCGGGG100.0070871348145.0145
GAGCGAA2100.0141.547621
GAGCAAA11500.0135.543471
AGCAGGC4850.0103.144338
CAAAAGC16150.096.068114
AGTAGAA3600.094.652781
GCAGGCA5450.093.119269
AAAAGCA16550.092.870095
GCAGGTC8200.091.951229
AAGCAGG20200.090.804467
GGATCTA259.376965E-486.999991
AGCAGGT11000.085.022738
AAAGCAG21900.083.755716
GTCATAC353.4313165E-582.857141
TCATACT353.4313165E-582.857142
GACAGTA1000.079.749997
GCAGGGG2000.079.749999
Produced by FastQC (version 0.11.2)