FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2004_day_1_ll-e.35200000088847.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2004_day_1_ll-e.35200000088847.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109621
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA5840.5327446383448428No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC4600.4196276260935405No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA3050.2782313607794127No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA2960.2700212550514956No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA2590.23626859817005866No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT1990.1815345599839447No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1800.16420211455834194No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1710.15599200883042483No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC1700.15507977486065627No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA1640.14960637104204486No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1560.14230849928389633No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT1530.13957179737459063No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1410.12862498973736783No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA1380.12588828782806213No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1370.12497605385829358No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1300.1185904160699136No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA1260.11494148019083936No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT1140.10399467255361655No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA1130.103082438583848No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG1110.10125797064431084No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG1110.10125797064431084No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCA100.0070823114145.09
GCAGGTA100.0070823114145.09
GAGCGAA1450.0105.000011
GAGCAAA7450.084.664431
AGCAAAA7150.084.160842
GCAAAAG7600.080.1315843
GCAGGTT554.3579348E-879.0909048
GCGAAAG1950.078.076933
AGCGAAA1950.078.076932
AAAGCAG10000.075.46
CAAAAGC8100.073.3950654
CGAAAGC2100.072.54
AAAAGCA8550.069.5321665
GCAGGAT807.998096E-963.43759
GCAGGAG2400.063.43759
CTAGACT502.0023213E-458.04
ATAAGAC502.0023213E-458.03
AGCAGGA3550.057.1830988
GAAAGCA2750.055.3636365
GCAGGGG3900.053.910269
Produced by FastQC (version 0.11.2)