FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2003_day_1_ul-b.3520000008887d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2003_day_1_ul-b.3520000008887d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140128
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA8240.5880337976707011No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC7710.5502112354418818No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA5630.4017755195250057No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA5060.3610984242977849No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA4870.3475393925553779No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA3090.2205126741265129No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC2890.2062400091345056No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA2880.20552637588490524No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA2820.20124457638730303No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT2780.19839004338890157No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT2660.18982644439369717No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG2600.18554464489609498No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA2570.1834037451472939No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA2180.15557204841287967No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA2100.14986298241607673No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA2100.14986298241607673No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC2050.1462948161680749No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT2040.14558118291847455No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA2000.14272664992007308No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC1910.1363039506736698No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA1850.1320221511760676No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC1840.13130851792646722No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG1790.1277403516784654No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG1790.1277403516784654No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA1770.12631308517926468No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC1710.1220312856816625No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG1690.12060401918246175No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT1670.11917675268326101No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1640.11703585293445992No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT1620.11560858643525919No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC1610.11489495318565883No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1580.11275405343685774No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT1560.11132678693765699No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG1540.10989952043845626No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG1540.10989952043845626No Hit
GCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACT1520.10847225393925554No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT1510.10775862068965517No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC1470.10490408769125371No Hit
GTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCT1470.10490408769125371No Hit
GGTTAATCTATTTTTATTAAATATATTTATTGTCATGGTTGGGCTTGAGT1430.10204955469285223No Hit
TCCCAGGACTTTGGAATCATGACCTGAGCTGAAGGCAGACACTTAACAAC1420.10133592144325188No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT1420.10133592144325188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1650.0105.4545441
AGCAAAA8050.085.5592
AAAAGCA9200.074.076095
GAGCAAA9600.074.0104141
GCAAAAG9400.072.53
AGCGAAA2250.070.888892
AAAGCAG12100.069.5041356
GCGAAAG2300.069.347833
CAAAAGC10250.066.487814
GCAGGAG2100.065.5952459
AGCAGGA3750.063.7999958
ACTCGGC350.003560107262.1428575
CGAAAGC2600.061.3461534
GCAGGAT1300.061.3461539
AAGCAGG16450.051.1246227
GCAGGGG3850.050.844159
AGCAGGT2250.048.3333368
ACCATGC604.930437E-448.3333328
GAAAGCA3800.041.9736825
CAATTGT951.02437145E-438.15789145
Produced by FastQC (version 0.11.2)