FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2002_day_1_ul-b.35200000088a1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2002_day_1_ul-b.35200000088a1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141606
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA4690.331200655339463No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA3960.2796491674081607No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC3590.2535203310594184No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA3420.24151519003432056No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC2910.20549976695902716No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT2710.19137607163538267No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT2410.17019052864991596No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT2400.16948434388373373No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2320.16383486575427597No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA2240.15818538762481815No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC2080.14688643136590257No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1990.14053076847026255No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG1960.1384122141717159No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG1800.12711325791280031No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1740.12287614931570695No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1660.11722667118624916No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1650.11652048642006696No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1640.11581430165388473No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA1590.1122833778229736No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC1500.10592771492733358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1450.095.01
GCGAAAG2800.082.857142
AGCGAAA2800.082.857141
CGAAAGC2900.080.03
GAGCAAA7500.078.31
GGAGTAC300.001932300472.59
GGTGAGA300.001932300472.58
GCAAAAG8350.071.197613
AGCAAAA8450.069.497042
AAAAGCA8600.068.284885
CAAAAGC9200.064.619574
TAGTGGC502.0043296E-458.09
AAAGCAG12650.057.3122526
GAAAGCA4400.052.7272764
AGGGATG701.6885378E-551.7857135
GATTAGC752.5394102E-548.3333368
TATGCTA450.00962218748.3333325
GCAGGTG450.00962218748.3333328
TTACCCC604.930616E-448.3333329
GATTAGG450.00962218748.3333324
Produced by FastQC (version 0.11.2)