FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2002_day_1_ll-e.35200000088996.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2002_day_1_ll-e.35200000088996.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43533
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA2530.5811683090988445No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC2370.5444145820412101No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2090.4800955596903499No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA1400.32159511175430133No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT1380.31700089587209707No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA1140.26187030528564537No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG1040.23889922587462384No Hit
GAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAAT1030.2366021179335217No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA1020.23430500999241954No Hit
GAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTA910.20903682264029588No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT850.19525417499368294No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA850.19525417499368294No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA800.1837686352881722No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC680.15620333999494634No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT680.15620333999494634No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA680.15620333999494634No Hit
CCCCACTCAGCAGGGAGCCCAATGTGGGACTCGATCCCAGGACCCTGAGA660.15160912411274205No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA640.14701490823053776No Hit
GGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAG630.1447178002894356No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC630.1447178002894356No Hit
CTTAACCACTCAGACACCCAGGCATCCCAACATTAAACAGATTTTTACCT590.135529368525027No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT590.135529368525027No Hit
GAGCAAAAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCA570.13093515264282268No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA570.13093515264282268No Hit
TTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCG560.12863804470172052No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC560.12863804470172052No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA550.1263409367606184No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG550.1263409367606184No Hit
GCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACT550.1263409367606184No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA530.1217467208784141No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC520.11944961293731192No Hit
AGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCAC500.11485539705510762No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC500.11485539705510762No Hit
AACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATC490.11255828911400548No Hit
AGCGAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAATA480.11026118117290333No Hit
GTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCT480.11026118117290333No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA460.10566696529069901No Hit
GACAATCGACTGAGCCACCTAGTTGCCCTGGTTAATCTATTTTTATTAAA460.10566696529069901No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT460.10566696529069901No Hit
GCCCAATGTGGGACTCGATCCCAGGACCCTGAGATCATGACCTGAGCCGA460.10566696529069901No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG450.10336985734959686No Hit
GCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAATAG440.10107274940849471No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT440.10107274940849471No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC440.10107274940849471No Hit
GAGCGAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA440.10107274940849471No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA440.10107274940849471No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC440.10107274940849471No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGT100.007067731144.999988
GAGCGAA600.0132.916671
ACTCCCT203.8409745E-4108.74999145
AGCAAAA2950.095.847462
GAGCAAA3200.095.156241
AGCATGC259.32607E-487.08
GGTATTC259.32607E-487.0145
GCATGCA259.32607E-487.09
GCAAAAG3800.076.315793
TAGCCCA300.001923275872.5145
AGCGAAA1200.072.51
AAAAGCA4250.069.941185
CAAAAGC4400.067.5568164
AAAGCAG5750.065.5652166
CGAAAGC1350.064.444443
GCGAAAG1350.064.444442
GCAGGGG2350.061.702139
CAGGGAA606.697148E-660.4166689
GTATATA651.0762586E-555.7692341
ATATAAG400.006012205554.3749963
Produced by FastQC (version 0.11.2)