FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_pr8_positive_control_nw_cb7-9.3510000008874b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_pr8_positive_control_nw_cb7-9.3510000008874b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102194
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG5840.571462121063859No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA5560.5440632522457287No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT3750.36694913595710116No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT3750.36694913595710116No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2830.276924281268959No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA1840.18004970937628434No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA1800.1761355852594086No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1780.17417852320097071No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG1720.1683073370256571No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1700.1663502749672192No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1650.16145761982112453No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT1650.16145761982112453No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1600.15656496467502984No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1590.1555864336458109No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA1370.1340587510029943No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG1360.13308021997377537No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1310.12818756482768068No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA1300.12720903379846174No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1280.12525197174002387No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT1260.12329490968158602No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1220.11938078556471025No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG1220.11938078556471025No Hit
GCATCCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTT1220.11938078556471025No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT1220.11938078556471025No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1130.11057400630173982No Hit
CTGTTAACCTGCTCGAAGACAGCCACAACGGAAAACTATGTAGATTAAAA1120.10959547527252089No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1070.10470282012642622No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1060.10372428909720727No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1050.10274575806798833No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACCC100.007081611145.0145
GAGCGAA850.0145.01
AGCAGGC253.6872734E-8145.08
GCAAAAG3950.099.113923
AGCGAAA1300.094.8076862
GCAGGGT2400.090.6259
TTGTCAA259.3624654E-487.07
TATACTA259.3624654E-487.05
ACTTGTC259.3624654E-487.05
AAGCAGG6150.083.699187
AGCAAAA5100.079.607852
AGCAGGG3900.078.076938
CAAAAGC5050.077.524764
AGGGTGT406.63461E-572.56
AAGGGTG406.63461E-572.55
TATTGGT406.63461E-572.59
ACTATTT300.001930753572.55
GAGCAAA6000.067.666671
ACAAGGG554.035579E-665.909093
CAAGGGT554.035579E-665.909094
Produced by FastQC (version 0.11.2)