000000000-ATLLR_l01n01_ny238_plasmid_mix2_lp_cb7-9.35100000088af5.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-ATLLR_l01n01_ny238_plasmid_mix2_lp_cb7-9.35100000088af5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	71972
Sequences flagged as poor quality	0
Sequence length	151
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG	216	0.30011671205468793	No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG	191	0.26538098149280276	No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA	174	0.24176068471072082	No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT	147	0.20424609570388486	No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC	144	0.20007780803645864	No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG	143	0.1986883788139832	No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT	134	0.18618351581170456	No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG	125	0.17367865280942588	No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC	123	0.17089979436447508	No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT	118	0.16395264825209804	No Hit
CCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGG	115	0.15978436058467183	No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT	112	0.1556160729172456	No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG	111	0.15422664369477018	No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT	109	0.1514477852498194	No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT	105	0.14589006835991775	No Hit
CTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCC	98	0.13616406380258989	No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG	98	0.13616406380258989	No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA	97	0.1347746345801145	No Hit
ATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTT	95	0.13199577613516367	No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC	95	0.13199577613516367	No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT	93	0.12921691769021287	No Hit
ACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATA	86	0.11949091313288503	No Hit
AACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAG	85	0.1181014839104096	No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT	85	0.1181014839104096	No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT	83	0.11532262546545878	No Hit
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCG	82	0.11393319624298338	No Hit
CTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCT	81	0.11254376702050797	No Hit
ATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT	80	0.11115433779803256	No Hit
ATTCTAGACGGGCCGGCCCCTGCGTGTGGCACGGGCGGCCGGGAGGGCGT	80	0.11115433779803256	No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC	79	0.10976490857555717	No Hit
AAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGT	78	0.10837547935308176	No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG	78	0.10837547935308176	No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA	77	0.10698605013060634	No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG	77	0.10698605013060634	No Hit
ACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGA	76	0.10559662090813093	No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT	76	0.10559662090813093	No Hit
TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT	73	0.10142833324070472	No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC	72	0.10003890401822932	No Hit
GACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGT	72	0.10003890401822932	No Hit
CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTT	72	0.10003890401822932	No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT	72	0.10003890401822932	No Hit
GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTC	72	0.10003890401822932	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCTCTA	25	9.351126E-4	87.0	9
ATTACCC	35	0.0035530685	62.14286	145
ATCATCG	35	0.0035530685	62.14286	8
CACACGG	65	1.08342065E-5	55.769234	5
AATCATC	40	0.006028177	54.375	7
CAGTTCA	40	0.006028177	54.375	145
TCTAGCA	40	0.006028177	54.375	2
ATGTAAT	40	0.006028177	54.375	3
TACTGAT	40	0.006028177	54.375	7
TAGATGC	40	0.006028177	54.375	5
TGTAATC	40	0.006028177	54.375	4
CACGGAG	70	1.6801683E-5	51.785717	7
ACGGAGC	70	1.6801683E-5	51.785717	8
TAGAGAG	45	0.009603095	48.333332	2
ATGCTCT	45	0.009603095	48.333332	8
TACTTTA	100	2.6987582E-6	43.5	7
ATATATA	100	2.6987582E-6	43.5	2
CATATAC	85	5.2920346E-5	42.64706	3
CTTTAGA	105	3.7747304E-6	41.42857	9
TATACTT	105	3.7747304E-6	41.42857	5

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content