FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_negative_rt-pcr_control_l_cb7-9.35100000088adb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_negative_rt-pcr_control_l_cb7-9.35100000088adb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTGAC21.550387596899225No Hit
GCAAAAGCAGGGGGAGGGTCAGGCAGAAGGAAAAGCAGGCTCCCTGCTGA10.7751937984496124No Hit
GAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAAAAAAATGAACT10.7751937984496124No Hit
CCGATGGTGCGCGATGCCACGGCGCTCGTGGTCGGCGACCGGCTGACCGC10.7751937984496124No Hit
CAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAG10.7751937984496124No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC10.7751937984496124No Hit
ATGCCACTCTGCTGCATTTGAAGATTTAAGAGTATCAAGTTTCATAAGAG10.7751937984496124No Hit
CCCCAGTTCCTGTTCGACCGATGCATCGAGCCGGTGGCGCTGCGTGTAGT10.7751937984496124No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC10.7751937984496124No Hit
CATCACCTTTGCCGTCTGAGTCCTTCAATGGTGGAACAGATCTTCATGAT10.7751937984496124No Hit
GAGCAAAAGCAGGGATGAAATAGGGAAGCAGTCAGAGGACAGCTCACATG10.7751937984496124No Hit
GAAAAGATGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCT10.7751937984496124No Hit
GGCGAATGAATGCCACTCTGGGAGCAGATCTGCATTCCAAGGGCTGAAGG10.7751937984496124No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAATAGC10.7751937984496124TruSeq Adapter, Index 25 (96% over 27bp)
AGCTTGATTTCCTTCGGGTTGCGCAGGAACTGCTGGCTCAGCTTTGCGAT10.7751937984496124No Hit
ATCTCAGGGTCCCAGGATGGAGCCCCATATTGGGCTCTCTACTCAGTGGG10.7751937984496124No Hit
CCATGGGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCA10.7751937984496124No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG10.7751937984496124No Hit
TGGGAAGAGAAGGAAGGAGGGGGGGAGGAAAAAAGAGAAGGAAAGGGGAA10.7751937984496124No Hit
GCATTTGCGGTGCTGCCTTCCTCTCCTTTATTGCCCGGGCTCAGGTCTGG10.7751937984496124No Hit
ATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGT10.7751937984496124No Hit
CAGCAGCTTGAAGCGTTCGTCGGTCGTGAACAGGTCGGCGCCGTCGCGAT10.7751937984496124No Hit
GTTTAGTCTATTCTTTCTTTTTCTTTTTTTTTAATAGTTTATTTATTTGA10.7751937984496124No Hit
CGTGATGCCGATCACGGCCGAGCTCCACGCGATCGCCTTCACGCGGCCGT10.7751937984496124No Hit
GGATACACCATGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCAT10.7751937984496124No Hit
AGAGGAATCCAGCGAGACACTTAGAATGACAATTGCATCTGTACCTACTT10.7751937984496124No Hit
TTGTATGGATAGTTTCTGTCTTCAAGTGCCTTGAGCTTTTCTTTTATGGT10.7751937984496124No Hit
CATAATAAGTGTTTTAAAGTTCTTGTCTACTAATTCCCTATTTGTCACTT10.7751937984496124No Hit
TTCTTATAACTTCTGTTCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA10.7751937984496124RNA PCR Primer, Index 18 (96% over 30bp)
TCCTTACTGTGCGTTTTGTGCTGCCTATATTGTACACAGAGACCAGTGCA10.7751937984496124No Hit
TGTATGGGTTCATTATAAAAGGAAGGTCTCATTTGAGAAATGATACTGAT10.7751937984496124No Hit
GTGGTGAGCCGAGATTGTGCCATTGCACTCCAGCCTGGGTGACAGTGCGA10.7751937984496124No Hit
CTCTCCCCACTCCCCCTGCTTGTGTTCCTTCTCTGACTGTGTCTGTCAAA10.7751937984496124No Hit
CTACCTGTTCTTTGTATGGATAGTTTCTGTCTTCAAGTGCCTTGAGCTTT10.7751937984496124No Hit
ACCCAAGGGACGTGCTGGGAGCAGATGTACACTCCAGGAGGAGAAGTGAG10.7751937984496124No Hit
CTAAAGTTATATTTAATTTTTTTTTTTAAGCTTTTTTCCTATCTGTTCTT10.7751937984496124No Hit
CCCCTGTCCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA10.7751937984496124RNA PCR Primer, Index 23 (96% over 30bp)
GAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAAGGTATATA10.7751937984496124No Hit
GTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTTTGATTATGGCC10.7751937984496124No Hit
GCCTTTGGCTCAGGTCATGATCTGAGGGCCCTGGGATCGAGCCCCGCATC10.7751937984496124No Hit
CCACATTCAGCAGGGAGCCTGCTTGAGATTCTCTCGTTCCCTCTCTCTCT10.7751937984496124No Hit
ATATAGTATCTGGCCCAGGCATACTATAATTTGACTGTTAGCAGATTCTT10.7751937984496124No Hit
ATGACAAACATTCCAATGGAACCATTAAAGACAGGAGCCCATATCGAACC10.7751937984496124No Hit
TTGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGA10.7751937984496124No Hit
GTTCATAGGAGCATTATTCACAATAACCAAAAGGTGGGCACACCCCAAGT10.7751937984496124No Hit
GCCTTGAATGGCATGTCAGGTAGGCCAGGAAACGAGGTGAACAAAATCAC10.7751937984496124No Hit
CCTCTAGCCCCACCCGCTGTCCCCTGCTCCTCATGCCCCCATGCTGTGTC10.7751937984496124No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGAGAAA10.7751937984496124TruSeq Adapter, Index 25 (96% over 27bp)
GGGATTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTG10.7751937984496124No Hit
ATGCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAAAT10.7751937984496124No Hit
CATATGATCGTTTGGTGCCTTGAGACGCCATGGCTTCGATGTCACTCATT10.7751937984496124No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA10.7751937984496124No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT10.7751937984496124No Hit
GGCCTAGAGCCTTGCTAGTTCTTTACTGTGTTTTATCACTGCAAATGTCA10.7751937984496124No Hit
TGCTGCACCTCATCTGACACCAGCTTCTCTGCTTTGCCCAAGGTCCCTAG10.7751937984496124No Hit
CCTCACGGAATCGGGTGATGGACGGTCACGAGCTTGGTGCCGTCGGGGAA10.7751937984496124No Hit
GATGTGGCCCCGAGTGTCAGGAATGTTTCTGTATCCCCAGGTGATTCTCC10.7751937984496124No Hit
CAACATTCCACGCTAACATGATTCAAATGATGTTTCCAGGATGCCCGGGC10.7751937984496124No Hit
GGCCATGGAGGTTGCTAATCAGACTAGGCAGATGGTACATGCAATGAGAA10.7751937984496124No Hit
GTCTGTTAAGTGTCTGCCTTCGGCTCAGGTCATGATCCCAGGGTCCTGGG10.7751937984496124No Hit
TAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTT10.7751937984496124No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATTCTGAGTG10.7751937984496124No Hit
ATCTCTATTTCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACAT10.7751937984496124No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC10.7751937984496124No Hit
GCTGTCCCCTGCTCCTCATGCCCCCATGCTGTGTCCCCTGGCCTCACTTC10.7751937984496124No Hit
GATCGAGCGACGAACCGGTCGCGTCACCGGCAATCCGCTGGTACGCGCCC10.7751937984496124No Hit
ATTTTATTCTGTGTTGATTTGTTTTCTGATTTTACTAGGGTAATATCCGT10.7751937984496124No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT10.7751937984496124No Hit
ATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAACTACC10.7751937984496124No Hit
AGTAGAAACAAGGAAAGAAACGAAAGGCAAGATAAGAAGGTCAGAACTTT10.7751937984496124No Hit
TTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAG10.7751937984496124No Hit
GTGATGACGCGGCCGTATGCGTTGAACGGCTAGCGATACAGGCCGGCGTT10.7751937984496124No Hit
TCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCCTCTCTCTGCATCT10.7751937984496124No Hit
GATGGGCAGAGAGAGAGGGAGAAGCAGGCTTCCTGCTGAGCAGAAAGACC10.7751937984496124No Hit
ACAGAGGTTGTGGTGAGCCGAGATTGTGCCATTGCACTCCAGCCTGGGTG10.7751937984496124No Hit
GCTGGGTCCCCCCTGAGCGGGGAGTCCGGTGCGGGACTTGATCCCTGTCT10.7751937984496124No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCCTGAAAAAAACACTCGCGCGATC10.7751937984496124RNA PCR Primer, Index 13 (96% over 26bp)
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT10.7751937984496124No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAATTTTTTT10.7751937984496124TruSeq Adapter, Index 25 (96% over 27bp)
TCCCACCTCTGGGTCACGCAGCAGGGATCTGGTAGGAACGGGAACAGGGG10.7751937984496124No Hit
GGATACACCATGGACACAGTAAACAACCAATATTCGAGAAATTTTTCCCT10.7751937984496124No Hit
AGCAGGCTATGGGGCAATCATCAGGTTTCTTATAAAACAATATGGAACAG10.7751937984496124No Hit
CTCATGGACACTACATTAAATAGAGTAAGTCAGACAGTGAAAGACAATTA10.7751937984496124No Hit
GTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATA10.7751937984496124No Hit
GAGCAAAAGCAGTCAGAGTGGCAGGCAGAGGCAGAGAGAGAAGCAGGCTC10.7751937984496124No Hit
GCATTATTCACAATAGCCAAAAAGTGGAAGAAAAAGTGTCTATCAACAGA10.7751937984496124No Hit
AAGTGGGGGCAAGAATACTGACATCAGAGTCACAGCTGGCAATAACAACA10.7751937984496124No Hit
CCTTCGGCTCAGGTCATGATCCCAGGGTCCTGGGATCGAGCCCTGCTTCA10.7751937984496124No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC10.7751937984496124No Hit
CCACTGAGCTGCATCACTAACTAACGAAAGAAGACTGCAGGAGGAACAGG10.7751937984496124No Hit
GGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCACATTCTTTCATAA10.7751937984496124No Hit
GCTTTCGCTCCCCCCGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAA10.7751937984496124No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC10.7751937984496124No Hit
CAATTAAAATATTCAAGTTAATGTCAATGAAAAGAACATAAATAACTTTT10.7751937984496124No Hit
AAAGCAGGAGGGAGGTGGGACCCGTAGCGCTACTGCAGGGCTGCCTCGGG10.7751937984496124No Hit
AGCCTGTCTGCCTGAGTCCCACGGCCCAGGTCAGGCACAAGCAGTCTCCC10.7751937984496124No Hit
ATGGGGCAATCATCAGGTTTCTTATAAAACAATATGGAACAGCTGGGGTA10.7751937984496124No Hit
TCCCCGCAGGGAGGCCGTGTGCTGCTGAGCCCTTTGCTAGGAGTGGGTAG10.7751937984496124No Hit
GGTCAGCCTGGCTCAGGATATGCTAGTTTGGCTGCAGAGGGTCCTACTAA10.7751937984496124No Hit
CGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGT10.7751937984496124No Hit
ACCCAATCCATTGTCTGCAAATCGCTTGCGGATATGCCAAAGGAAACAGT10.7751937984496124No Hit
CCCCTGCTCTCTACACTTCAAAGATTTCTGCTATTAGAATAAGGTTCTGT10.7751937984496124No Hit
CATGAGTGTCTCCAAGATGCATATCTTTGTTTTTAATTCCAGTGTAATTA10.7751937984496124No Hit
GGCTGTATGGAGGATCTCCAGTATAAGGGAATGTGGTGCTTATGGCATTT10.7751937984496124No Hit
GTGGCTCAGTCGTCCAGTGTCTGTCTTCAGCTCAGGTTGTGATCCCAGGG10.7751937984496124No Hit
GGACCTGTGTCACTCCCCTGATGTGGCCCCCCGACACCTTCATGCGGTAA10.7751937984496124No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA10.7751937984496124No Hit
CCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAACTATCCATACAAA10.7751937984496124No Hit
GGATGGCAGCCGCGCGATGCGCACGCCACTGCCGAGATGGGCATGCGTCG10.7751937984496124No Hit
GATTATGGCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGC10.7751937984496124No Hit
GCACTTGGTAGCCACGTGTGGTGGCTACGTGGTCCTAAAACAGGGTGGCC10.7751937984496124No Hit
TGGTGGAACGACCACGGCTCGCAGCGGCTGATGGAATTTGACCTCGGTGC10.7751937984496124No Hit
GAAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCG10.7751937984496124No Hit
GTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTCTGCATTGTCT10.7751937984496124No Hit
ATTTGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGGACAGGGATGGTAG10.7751937984496124No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG10.7751937984496124No Hit
CCAGGGGGTTCGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATCT10.7751937984496124No Hit
AGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT10.7751937984496124No Hit
GATGTTGGAGACCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGATC10.7751937984496124No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT10.7751937984496124No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTT10.7751937984496124No Hit
GGCGGAAAGTGGATGAGAGAACTCATCCTTTATGACAAAGAAGAAATAAG10.7751937984496124No Hit
TAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATAT10.7751937984496124No Hit
TTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCT10.7751937984496124No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA10.7751937984496124No Hit
GAGGCCGTGTGCTGCTGAGCCCTTTGCTAGGAGTGGGTAGTCAGTGTCTC10.7751937984496124No Hit
GAATATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCGTATGCCGTC10.7751937984496124No Hit
GTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCAC10.7751937984496124No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA258.5156804E-529.045-49
Produced by FastQC (version 0.11.2)