FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_negative_extraction_control_nw_cb7-9.35100000088764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_negative_extraction_control_nw_cb7-9.35100000088764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172825
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGGTGTCCACACGGGCATGTTGGGTGTGGGGACAGCAGCTCTGC6510.3766816143497758No Hit
GTCCATACAGCGAGGACCATTTGCCTTAGGAAGGAACGGATCCCACAGGG5640.3263416751048749No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT4350.25169969622450455No Hit
GATTAAGAAGGCCCGGGTCACAGGGCTCCAGGCACCCCGTGCGGTCAGTC3780.21871835671922463No Hit
GAGTATGATGTTGGCAGCTTTGTCCCTCGAACTGGCCTGACTCTGCTGAG3480.20135975697960365No Hit
GTCCAGGTGTGGGCACAAGTCGGGGGTCAGCTTGCCTTGACTTCGGGCAT3290.19036597714451034No Hit
TCCTATGTCTCTCTTGCCCCGCGTGGGGAAGTCCTGTGTCAGGCCTTTGT3240.18747287718790684No Hit
GAATTAAGTTCCTCCCTTGCATGAGAATGGATCCCAACCGTGACAGCTGC3160.18284391725734125No Hit
TTCAAGGACAGATTAAGAAGGCCCGGGTCACAGGGCTCCAGGCACCCCGT3100.17937219730941703No Hit
AGATAATACAGGCCAAGAGGTTAGTGCAGAACCCACACTGTGGTTAAGGG3060.17705771734413425No Hit
AGCGAAAGCAGGGCATCTCAAAATCACCTGCAGGAACACGTCTGAAAATG3050.17647909735281353No Hit
CTTCCGTAGGAGCACGTACCTCAGTTGCTGGAACATTTTGCTCATGACTC3040.17590047736149284No Hit
GTCCTGCAGCTGTCACGGTTGGGATCCATTCTCATGCAAGGGAGGAACTT3000.17358599739621003No Hit
TCCCTGTGGGATCCGTTCCTTCCTAAGGCAAATGGTCCTCGCTGTATGGA2990.17300737740488933No Hit
GTATGGACAGACCACATGTCCCTCACACGTTCACCAGCCGAGTGGGCCTC2940.17011427744828583No Hit
TCCCTGAACTGTCTCATGGTGATGGGTGCCACGGAGTAGTTCTCTGCTCA2830.16374945754375814No Hit
GGTCTGGGCAGCTCCTGGGTCCTGCCGGGGATGGGAGGGCCACTGGGTAA2690.15564877766526833No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC2650.15333429769998555No Hit
CACCTGGACAACGATGTTGAAACCCTGAACCGACTGAACGCCCCAAGTCC2640.15275567770866483No Hit
GCCCCACACAGCCCTCGGCCTCAGCCTGAATCGACTGCTATGTGAGCTGC2580.14928395776074063No Hit
TTCACCAGCCGAGTGGGCCTCCAAGCCGTGCTGTCTCAGCAGAGTCAGGC2550.14754809778677855No Hit
TTCTTAATCTGTCCTTGAACGGTGAGCAGAGAACTACTCCGTGGCACCCA2460.1423405178648922No Hit
GTCTCATGGTGATGGGTGCCACGGAGTAGTTCTCTGCTCACCGTTCAAGG2390.13829017792564732No Hit
TCCCTCACACGTTCACCAGCCGAGTGGGCCTCCAAGCCGTGCTGTCTCAG2370.13713293794300593No Hit
GGGTAAGGTGATGCGGCAGCAACCATGTGACTATCAGGACACTTCCGTAG2350.13597569796036454No Hit
GTTCAGGGTTTCAACATCGTTGTCCAGGTGTGGGCACAAGTCGGGGGTCA2300.13308259800376102No Hit
CTGTATGGACAGACCACATGTCCCTCACACGTTCACCAGCCGAGTGGGCC2300.13308259800376102No Hit
GTCCACACGGGCATGTTGGGTGTGGGGACAGCAGCTCTGCCAACACACAC2280.13192535802111963No Hit
AGCGAAAGCAGGGTGTCCACACGGGCATGTTGGGTGTGGGGACAGCAGCT2200.12729639809055404No Hit
TTCCAGAGGCAGGTGTGTCCTCAGGATGCAGTAAACCCCATGCGCTGTTG2140.12382467814262983No Hit
GCCCAGACCCCATGCCCGAAGTCAAGGCAAGCTGACCCCCGACTTGTGCC2130.12324605815130912No Hit
AAGCAGGACCAGGCCCTGGGTGAAGTGGCCTCCTCCTTCCAGAGGCAGGT2100.12151019817734704No Hit
GTATGATGTTGGCAGCTTTGTCCCTCGAACTGGCCTGACTCTGCTGAGAC2100.12151019817734704No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG2070.11977433820338493No Hit
GACATAGGAAGTGCCGGGACCCCCAGAGCAGTGTTTCCCTGAACTGTCTC2020.11688123824678143No Hit
CCATGAGACAGTTCAGGGAAACACTGCTCTGGGGGTCCCGGCACTTCCTA2010.11630261825546072No Hit
GTCCTTGAACGGTGAGCAGAGAACTACTCCGTGGCACCCATCACCATGAG1920.11109503833357443No Hit
TCCCCACGCGGGGCAAGAGAGACATAGGAAGTGCCGGGACCCCCAGAGCA1910.11051641834225372No Hit
GTACAGGAACCACGGGACCCCTTCTCTGTGTTCCACCTTGAATTAAGTTC1900.10993779835093304No Hit
CCCCAAGTCCATCTTTTCAGACACTGAGGGAGGCCCCTGAGAGCAGCTCA1890.10935917835961231No Hit
AATGAACAGTGACCGGTCACCAACAGTCAGTCTGTCCTGCAGCTGTCACG1850.10704469839432952No Hit
AGCGAAAGCAGGACCAGGCCCTGGGTGAAGTGGCCTCCTCCTTCCAGAGG1830.10588745841168812No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA1810.10473021842904671No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT1810.10473021842904671No Hit
GGCCCTGGGTGAAGTGGCCTCCTCCTTCCAGAGGCAGGTGTGTCCTCAGG1810.10473021842904671No Hit
TATCAGGACACTTCCGTAGGAGCACGTACCTCAGTTGCTGGAACATTTTG1810.10473021842904671No Hit
GTTGGTGACCGGTCACTGTTCATTTTTGCATTTTCTGAGTCATGAGCAAA1790.10357297844640531No Hit
CATCATACTCAGAGGGAGACAACGCGACTGACCGCACGGGGTGCCTGGAG1770.10241573846376392No Hit
GTTCAGGGAAACACTGCTCTGGGGGTCCCGGCACTTCCTATGTCTCTCTT1750.10125849848112252No Hit
ACCCTGAACCGACTGAACGCCCCAAGTCCATCTTTTCAGACACTGAGGGA1750.10125849848112252No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA100.007085824145.01
TGTAGAC100.007085824145.02
AGCGAAA1450.0135.01
CAGGCGG256.4496307E-6116.09
GCAGGTA203.8605882E-4108.752
CGAAAGC1950.0100.384623
GCGAAAG1950.0100.384622
GCAGGCG301.5956994E-596.6666648
CAAGGTA259.373517E-487.08
ACAAGGT259.373517E-487.07
ACAACAA300.001933024472.5145
CTGATCC300.001933024472.59
GTAGAAA851.6916601E-1068.235291
GGGCGTG350.00356151462.1428577
GGTACTG400.00604245354.3755
TAGGGCG400.00604245354.3755
AGGGCGT400.00604245354.3756
GAAAGCA3700.052.9054034
GTCTCGC553.2115428E-452.7272769
AGTATGT553.2115428E-452.7272768
Produced by FastQC (version 0.11.2)