FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_library_prep_negative_control_lp_cb7-9.35100000088b03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_library_prep_negative_control_lp_cb7-9.35100000088b03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA10.7462686567164178No Hit
GTGGGGGACCGTGTAGGGCATCACGGCCAGATCGCGTATCGCGGCCGTCT10.7462686567164178No Hit
GATCGTGAAGAAGCCCGCGCCGTCGATGCGCGCGGCCTTCACGAGTTCGG10.7462686567164178No Hit
GAAAGGCAGTGTCGCGCCGTATGAACTGAAGGCGAACCCGGCCGCGCAGC10.7462686567164178No Hit
CCGGCCAGGTGGCCGAGGAACTGGTCGGCCGCATCGACGTGCACGACCTT10.7462686567164178No Hit
ATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAGGATCA10.7462686567164178No Hit
CTCGTTGTACGTGCTGGGCGCCTGGAGCTCCGACAGGTCCGGGCCGGGCG10.7462686567164178No Hit
GGAAGAATGGAAGTGACTTGCTCTCGGCCACCTCCTGCTTGGGGGACCTT10.7462686567164178No Hit
TGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGACATCCA10.7462686567164178No Hit
TCTATGGTCACTGTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGC10.7462686567164178No Hit
GAACAAGAGCGCTTGTTCGCACCGGAATGGATCCCAGAATGTGCTCTCTA10.7462686567164178No Hit
AGGCAGGAGTGATCCCTAAGCGTATGAAGAATCGGGCGCGACGACAACCA10.7462686567164178No Hit
GAGCGGTACCATTTCGTCGATCAGGTTCGCGTCGCCGACCCACTCGCGTG10.7462686567164178No Hit
GTAGAAAGGTGAGTCGGAAGCAGAGTTGCCAGGATGAGGCTGACAAGCAG10.7462686567164178No Hit
GTAGATGCGCAGGTAGTGATGGCTCTCGACGTGCGACGGCCCCCACACGT10.7462686567164178No Hit
ATCATCGGGCAGAGCACTGGATCGTCGTGAAGGGCACCGCCCTCGTCACC10.7462686567164178No Hit
AGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAG10.7462686567164178No Hit
GCCAGGGCACGAGTGTCGCGCTATATACCGTGAAACTGCCGTCGTGATCG10.7462686567164178No Hit
ACGTGTAGCAATCGACCATCGCGTCGTAGGTCCGCTCGGCTTCCGCGAAA10.7462686567164178No Hit
CCTCCATGTGGTGGTGGGACTGGGGCTAGGGGGCAGGGGAGACAGCCGGG10.7462686567164178No Hit
GATCTCGGCGGCGGCTACCAGGCGGTGTTCACGCTCGAAAGTGGCTTCAA10.7462686567164178No Hit
CAACTCACCGTGCGCGCGTAGTCCTTCTTGTCCTCGTAGGACGGGTCGAT10.7462686567164178No Hit
GCATGTAACAATTTGATAAGAGAGTATGCAGACCTCATGTTAAGTTTTCT10.7462686567164178No Hit
GAGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAA10.7462686567164178No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCTCATTGCCATCATCCACTTC10.7462686567164178No Hit
GAAACAAGGGTATAGGGGTCATGAGGTTATGGGAGATCAGAAGTCTGAAT10.7462686567164178No Hit
CACGTCGACCATCATGAACATCCCGGCTTCCGGCAGCAGGCAGCGCAGCC10.7462686567164178No Hit
CTATCAGCGTGTGATTTGCCGCATGTAGAAGATCGTCTGGATCGTCGGCA10.7462686567164178No Hit
GTCGAAACCTTCAGACTGGAAGCGAGATGAATTCCTCCCTGCAACCTGCA10.7462686567164178No Hit
GGCCCTTGGTCGGCAGCAGCCCGAGGTTCACGCCCATGTTGATGAACGTC10.7462686567164178No Hit
AGGTGAAGGAGTACGACCGTCAGTACATGTCGGGTCTCGTCACCGCGCAG10.7462686567164178No Hit
TGTCGCGATCCCGAATAGGCGAGCCCTCGGTGTAAACGCCTCCCGATGCC10.7462686567164178No Hit
TCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGG10.7462686567164178No Hit
TTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGAAACTCTGTTGTCCT10.7462686567164178No Hit
GCGACGACGTCGTCAGTTTGTGCACTTCGATGGACTTTTCGCGGTCGATC10.7462686567164178No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT10.7462686567164178No Hit
AACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTCAGGTC10.7462686567164178No Hit
TCGCATCCCCGTCTTTACCTGCCGTTCCTGAGCGTTGGCAAGGTCGTCGC10.7462686567164178No Hit
ATGCTCGGCAACTTCTCGTTCGGCGACTACTTCAAGCACGACGCGATCAA10.7462686567164178No Hit
GATGCCAGACCCGCATCCATGCCATCCCGCCCGTACGGCAGCGTACCGAC10.7462686567164178No Hit
GAGCAAAAGCAGGGGGACAAAAACATAATGGACTCCAACACCATGTCAAG10.7462686567164178No Hit
AGTTCTACCAGAACCCCGACAATCCGGCGGAGAAGCAGGCGCTGCTGCAG10.7462686567164178No Hit
CAAAGAACAATCGACAGCAGCCGCCGACAGTTCTGGGCTGTGCCAGATCT10.7462686567164178No Hit
GATATAGAATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTTCA10.7462686567164178No Hit
ATTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGTGAT10.7462686567164178No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT10.7462686567164178No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC10.7462686567164178No Hit
ATACTCGTCGTGACGATGCAGCACGCATCGCGCCACACGAATACAACGAA10.7462686567164178No Hit
AAACAAGGATTTAAGAAAGCCCGCATATTCAGGTTGACAGTTCCAGAGGG10.7462686567164178No Hit
CTATCGCGTCTGACCCCAGGCCGCGCGCCGCTTACTTGCCGGATTGACCG10.7462686567164178No Hit
GTCCATGGAGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAG10.7462686567164178No Hit
GTGTATGAGCTGCAGGCCAGTCGTGTGTCCAGTGATGTTATTGATCAGAA10.7462686567164178No Hit
GAACTGCAGCGCGTCCTGCTGCAGCCCGGCGATTGCCGCCGTGTTGCCCG10.7462686567164178No Hit
GCCTACTGAAGTGAAACAACAGCTGTACGTAGGCAGATTGGAGTGGAGAA10.7462686567164178No Hit
CCTTCATCGCAAGCTGCAACGTCGGTAGCGCGAGCAGCACACTGCCGAGC10.7462686567164178No Hit
TTTTTTTAATAGTTTATTTATTTGACAGAGAGAGAAGGAAAGAGAGCAAA10.7462686567164178No Hit
ATGTCATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATT10.7462686567164178No Hit
TGGCAGGAATGGTATGGCGACAAGGAAACCGCGGTCTGCGTGAACAGCGG10.7462686567164178No Hit
TATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAATGCA10.7462686567164178No Hit
GCCCGAATGCGGCGATCGACAGGATCGACACGATCACGAGCGTGCGGCGT10.7462686567164178No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA10.7462686567164178No Hit
GAATTGCGGCGCGTCGTACGCCACCATCATCGTATACTGCCGGCAGGCGA10.7462686567164178No Hit
ATACAGCGCGCCGCCGCTCACGGCCTGCACGAATGCGCCGAGCAGGCCGG10.7462686567164178No Hit
CGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAA10.7462686567164178No Hit
CCTTCCGCGTAGACGAATTCCACGTCGACGCGGAAGGACTGGACATGAAG10.7462686567164178No Hit
GTTCGAAGACGACGTTCGGTTTCAGGTAGATCTGGGTCATGTCAGGAATG10.7462686567164178No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGTAAAAAAAAAAGAGGAAGCT10.7462686567164178TruSeq Adapter, Index 25 (96% over 27bp)
GAGATACGACGTGTTGGTCGCAAGCACCGCGCCCGGCTTGCACACGCGTG10.7462686567164178No Hit
GTCCACTTCAGAAATCAAGTTAAAATAAGGAGGAGAGTTGATACAAACCC10.7462686567164178No Hit
TTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAA10.7462686567164178No Hit
GTTATTGGTATTTTGATAGGTATTACATCAAATATGTAGATTTTGGGTAG10.7462686567164178No Hit
GCATGACGTGCGGCTTGGCGTGAGGTCCGCGGAAGTATTCAAGACCGCGT10.7462686567164178No Hit
ACGTGGATCGCCATCGCGGTCTGGCCGTCCTTGAAGGTCGTGAATTCCTG10.7462686567164178No Hit
GTGTACGGCCGCCTGCGGGTGGTTGCGTACGAGGACGGTTTCGTCGCCGC10.7462686567164178No Hit
GGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGAGAATTCAT10.7462686567164178No Hit
CATGAACATCCATTGCCAGCCGTGGAAGCCCGAGCCGCCGTGGAAGCGCT10.7462686567164178No Hit
GACGAGCAGCGTTCCGTTCATCACGTCGCCCTGAGGGTCCAGCGTCGGGC10.7462686567164178No Hit
CTTATAACTTCTGTTCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA10.7462686567164178TruSeq Adapter, Index 18 (96% over 30bp)
GAATGTTTGTGCATACCCACTTGGTTCATTATCCTCAGGTAGTGGTCCAT10.7462686567164178No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACTCTGT10.7462686567164178TruSeq Adapter, Index 25 (96% over 27bp)
CCCCGTATCCGTCCCGCATGTCGGCCTTGAGCGCCGGCCCCGGCGCATCC10.7462686567164178No Hit
TTCCTCAACTGTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCT10.7462686567164178No Hit
ACCTGTTCGTCGAGATCGGCGCGTATCGCGGCACTGCGTCGCCGGTAAAG10.7462686567164178No Hit
GCAAGATACGTTCCGCCCAGCGATAGGTATCGAGCGGGTTGATGTACTCG10.7462686567164178No Hit
ATGCCGGACAGGTTGATGCCGACCGACAGACCGAGGAAGTTGTAGAGGCG10.7462686567164178No Hit
ATCCAGAATAGCATAACAATAGAGAGGATCTCGTATGCCGTCTTCTGCTT10.7462686567164178RNA PCR Primer, Index 1 (96% over 26bp)
GGGATAGTTAGTAAGATTTAAAGATACTATTTGAAATGACAATGAATGCA10.7462686567164178No Hit
CTTTTATTCTCTCCATATTGAATATATTTGACCTGCAAAAGCAGGTCAAA10.7462686567164178No Hit
GCTGTAGTCACAACAGTCACACTTGAAGGGCCGGTCCTCGCTGTGGCGAA10.7462686567164178No Hit
TGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAA10.7462686567164178No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT10.7462686567164178No Hit
CGATCGGATGGCGCTCCGCGTGATACGTGTCGAGCAATCGCTCGGGCGTG10.7462686567164178No Hit
CTCCCTCCTCAGTGAAAGCCCTTAGTAGCATCAAGGTCTCTAATCGGTTA10.7462686567164178No Hit
GTACCGAGCAGGCCGCCCGTCGCCGGATTGCGGACTTCGAACGTCTCGCC10.7462686567164178No Hit
CCATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGT10.7462686567164178No Hit
ATCCCAGGGTCCTGGGATGGAGCCCAACATTGAGCTCTCTGCTCAGCAGG10.7462686567164178No Hit
ACATCCAAATGTGCACTGAACTCAAACTCAGTGATTATGAGGGACGACTA10.7462686567164178No Hit
GGCCTGTTGCTGCAGGCCGGCTATTTCGCGTTCACGTACCTGAGCCTGAA10.7462686567164178No Hit
TCAGAGAACAAGAGCGCTTGTTCGCACCGGAATGGATCCCAGAATGTGCT10.7462686567164178No Hit
CCCTCGAGGAAATGTCACGAGACTGGTTCATGCTCATGCCTAGGCAAAAG10.7462686567164178No Hit
ATGCCGAGCGTCAGGCGCGAACCGTCGATCAGCCACGAGAGGATGTCGCG10.7462686567164178No Hit
CGCTCGATGCGTTCGCGGCCGATCCGAACCGGTTGCTCGGACGCCTGCCG10.7462686567164178No Hit
GGAAACGAACAACCTGCGCGGCGGCGCGGGCCTCGTACAGGCCGTCGACA10.7462686567164178No Hit
CCGCTGCGCCGGTGCCAGCGGCCTGTCGAACGCGATGAAGTATCTGTGCG10.7462686567164178No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAGGG10.7462686567164178TruSeq Adapter, Index 25 (96% over 27bp)
CTATCGCGTGGCCCGCGCGGCGATCCGCCACGACGTGCGCCACTTCGTGC10.7462686567164178No Hit
CACTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTG10.7462686567164178No Hit
GCAGTTAAAGTCAGGGATTTGGGGGCACCTGGGTGGCTCAGTTGTTAAGT10.7462686567164178No Hit
TACCAATCTCGCTCCCGGGACGGTGTCGGCGGCAAGCACTGACGCGGTGA10.7462686567164178No Hit
CAGTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATG10.7462686567164178No Hit
CAATAAGTCTAGGGAACATCTGTCACTTTACATTGTTACAAAAGAAAAAA10.7462686567164178No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG10.7462686567164178No Hit
ATCTGGTGCGGGGCACTGGCCGATTCGACGAAGAACTGCATCAGTTCGCC10.7462686567164178No Hit
GCACTCCCAGGACTGACCAGCGGGCGTGATTGCTCGACGAATAACGAAGT10.7462686567164178No Hit
CTGGTGGGGAGCGCCAGGATGCCACAGAAATCAGAGCATCTGTCGGAAGA10.7462686567164178No Hit
TTCTAGGCCCACGACGGCCGCGTGGACAGGGCACTTTATCATCGGGCTGT10.7462686567164178No Hit
AGCAAAAGCAGGTTGCCAACTCCGCTTTTGTGGAGCGAGTGCGAAAGCGG10.7462686567164178No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10.7462686567164178No Hit
GCTTACGCAAACGTCGTGCCATGTCGCTGCAAATCCGGCCCGCAGCCCCG10.7462686567164178No Hit
GGCCGTGATCGGCTTCTGGACGGCCGCGCTGATCGTCGCGGAAGGCATCC10.7462686567164178No Hit
GCCCAACCATGACAATAAATATATTTAATAAAAATAGATTAACCAGGGCA10.7462686567164178No Hit
AATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCATGTTCA10.7462686567164178No Hit
GCCGACGCGCGAGTTGCAGCCGGGCGTGCAGTTCCTGCATTGCCTGGCCA10.7462686567164178No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG10.7462686567164178No Hit
ACAAGACCCTGCTCGTTCACCTCGACGACAGCGAGCGCTGTGCGACGCGA10.7462686567164178No Hit
GGTCGGCTGCACGATGTCGTCGTCGCGATGCAGCGCGCTGCGCGGCAGGT10.7462686567164178No Hit
ATTTTCTTCACGCGCGGCTTCTGCGTCGCATAGCGGAGGAAGCGAAACGT10.7462686567164178No Hit
ACGCAAGAGCGCATTGCGCCAGAAATCCTGTTTGGGCAGTTCGGTCAGCC10.7462686567164178No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA10.7462686567164178No Hit
ACATCAGCAGCGTGCGTTCGGCGTCGAGCGCGGCCAGCGCCGACAGTGTG10.7462686567164178No Hit
TTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGAC10.7462686567164178No Hit
GTCGACGGGCCAGCAGATCAAGCACCTGTTCGCGCTCGAAGACCGCCACC10.7462686567164178No Hit
GATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCACTGAGCCACCCAGG10.7462686567164178No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA10.7462686567164178No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)