FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_extraction_control_l_cb7-9.3510000008897f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_extraction_control_l_cb7-9.3510000008897f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15732
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTAGAGAGGGAACACGAGCAGGGTGAGTGGGAGAGGGAGATGCAGGC1400.889905924230867No Hit
GTTGAAGGGTTTGCATTCTCTCCCTGGGTTCAGTCTCTCTCTCAGCACCC680.43224002034070685No Hit
CCTCTAAGCAGACTCCCTCCTGAGCAGAGAGCCCAATGCAGCCAAAGGCA610.38774472412916344No Hit
ATATAATGTGAAGTGAGAAGGCAGACTTCCCAACAGTGTGCAAAACAACC580.3686753114670735No Hit
CCATTGATGTATTGGGGAAAAAAAATCACTGTGGGAAAGAAAGCCTGGGT540.34324942791762014No Hit
ATTTAGACATTGAACAGATCATCTTTGCTAACCGTTGGTATTTGGTGGGT500.3178235443681668No Hit
TTATATAACAGCCCACAAATATTTTTCAAAACATGGCAAAGGGATCTGAC480.30511060259344014No Hit
GGGTAAGGATGGTTTCTGCCTAGAAAACCAGGACTTCAAATTTGCAGGGA450.2860411899313501No Hit
TCCCAACAGTGTGCAAAACAACCTCATTTTCATTATAAGACATACCACAT440.2796847190439868No Hit
GTATTACTCATTTCCTGTGAATGACTGTTGTAGATCTTCATCCAAAGATG420.26697177726926014No Hit
TCTGAATGCTTCCCCATCTTTAAAATGGGAATAACGAGGGGCGCCTGGAT410.2606153063818968No Hit
ATATAAACTTGAGCCCCTTTGCTTTACACTGTTGAAGGGTTTGCATTCTC400.25425883549453343No Hit
ATGTATAAGTAGCTTGTGTAGCTGTTTTTTTCTTTCCTTTCCTTTTTAGG370.23518942283244343No Hit
AGTTTATATAACAGCCCACAAATATTTTTCAAAACATGGCAAAGGGATCT360.2288329519450801No Hit
TCCTTACCCAGGGTAACCACAGTTACTTCTTTATTAGTTGACTTTCTCTG360.2288329519450801No Hit
AGCGAAAGCAGGGGGAAGTGGCAGGGAGAGGGAGAACGAGGCTCGTGCTG350.22247648105771675No Hit
TCTCAGAGCTTACCCTCAGGAAATGAGTGGAACATGCACATGGCTACAGT350.22247648105771675No Hit
ATTTATAAGAACCTTGTGTATGATGAAACAAGTTTGGAATGAGCTAAACT340.21612001017035343No Hit
CCCTAGTACTGTAAAAGCTTCTACCGACACTACCGCTCTATCCTGCCATC340.21612001017035343No Hit
AGGTACACATACTCAACAAGGTTTTTAAGAATCCACAATGAGGGTTGCTG340.21612001017035343No Hit
CTTCTACCCTTTGTTACATTCTTAAGAATGGAAATCCGTTGTTATTGTAA340.21612001017035343No Hit
GTTGTAGATCTTCATCCAAAGATGCAGTACTTCACTGGGTCACTATTGCA330.2097635392829901No Hit
GTATTGACCAGAAGTGTGATTTCCTAGCTTTAGGAATTTGTACAGCCCCA320.20340706839562672No Hit
GTAAAGGACAGTAGTTAAATTTCCATGTCAGATGGAAATGTTTTGAATTT320.20340706839562672No Hit
GAGTAATACTGCTCCCATCTCCAATTTACAGCCCTGTGAAAGCCGTCAGT310.1970505975082634No Hit
GTTATATAAACTTGAGCCCCTTTGCTTTACACTGTTGAAGGGTTTGCATT310.1970505975082634No Hit
GTATACTATGCAGCACTTAAATGACACAAGGTACACATACTCAACAAGGT300.19069412662090007No Hit
CAATATACCCTTTGACACAGAAATTCTGCTTCTCAGAGCTTACCCTCAGG300.19069412662090007No Hit
CCCACCAGCAATGGGAAAGGATGCCTATTTATTTCATCATCCAGTTAACA290.18433765573353675No Hit
TCTGTAGACTGTAGTTTTGAGAACAGAGTTGCTAAGGGTCTCAGAGGGGT290.18433765573353675No Hit
AATATGTACAGAGAAATTGACCTTTTAAATCATTCTTTCCTCTAAGCAGA290.18433765573353675No Hit
TTATAAGACATACCACATAACCAAACTATTGATCAAGGTTGTACCTGCAG290.18433765573353675No Hit
TATTTGGGCCGTGACAGGCAGGAATGAACTTGTCAAAAGTCACCCCTCTG290.18433765573353675No Hit
CCTTTATACCATGCTCCAGATAGGGTGCTGAGAGAGAGACTGAACCCAGG290.18433765573353675No Hit
ATCTGGAGCATGGTATAAAGGAGAGTTTAGGGGAGGGCCTGATTTGAAGA280.17798118484617342No Hit
TTTTAAGCCCATGAAAAGGAATTTCAGAATGAAGTCTGTAATTCCAACAA270.17162471395881007No Hit
ATCTAATAGTCAATTTATCTGATAGTTATTAAGATACGAAATATCTGAAG270.17162471395881007No Hit
CAATGAGGGTTGCTGGGTAGCTCAATCGTTAAGCGTCTGCCTTCAGCTCA260.16526824307144672No Hit
GGTTAAGGTGTCCTGTGGTCTCTCATTAGAGAGACCCTGGCCCTAGTACT260.16526824307144672No Hit
GTACAGAGAAATTGACCTTTTAAATCATTCTTTCCTCTAAGCAGACTCCC260.16526824307144672No Hit
GCTTTACACTGTTGAAGGGTTTGCATTCTCTCCCTGGGTTCAGTCTCTCT260.16526824307144672No Hit
CTCCATTGATGTATTGGGGAAAAAAAATCACTGTGGGAAAGAAAGCCTGG260.16526824307144672No Hit
CTAGATATCTGCTATTGTCTTCTCAACCAATCCCAATCAAGGGTCCATTC260.16526824307144672No Hit
TTATAGGGATGTGCAGTCCTGCCCCCTTTCCTGTCTACATTTTCTGTCCC250.1589117721840834No Hit
GGCTTTAACTGCAGGTAAATTGTGCCTTAAAAAGTAGATAAAGGAGAAAA250.1589117721840834No Hit
GTACACATACTCAACAAGGTTTTTAAGAATCCACAATGAGGGTTGCTGGG250.1589117721840834No Hit
ATACTATGCAGCACTTAAATGACACAAGGTACACATACTCAACAAGGTTT250.1589117721840834No Hit
CTCTAATGAGAGACCACAGGACACCTTAACCACAATAATAGTTACTGACG250.1589117721840834No Hit
CCCTTCAACAGTGTAAAGCAAAGGGGCTCAAGTTTATATAACAGCCCACA250.1589117721840834No Hit
GTACTTCACTGGGTCACTATTGCACACGTATTTGGGCCGTGACAGGCAGG240.15255530129672007No Hit
GGGTAGAAGAAATGAAATCAAATTCACCTAGCAACAGAGAAAGTCAACTA240.15255530129672007No Hit
CCACAATATAATGTGAAGTGAGAAGGCAGACTTCCCAACAGTGTGCAAAA240.15255530129672007No Hit
CTCATTAGAGAGACCCTGGCCCTAGTACTGTAAAAGCTTCTACCGACACT230.14619883040935672No Hit
GGTAAATTGTGCCTTAAAAAGTAGATAAAGGAGAAAAATAATATGTACAG230.14619883040935672No Hit
ACCTTGGACAATTCAAGCTTCTGGGAAGTACTTTGGATTGTTGGAATTAC230.14619883040935672No Hit
ATCCTGCCATCTCCTCCCTCCTCTCTTCAAATCAGGCCCTCCCCTAAACT230.14619883040935672No Hit
ATCAAAATGTTTGCCAAACAAAACAAAAAATATTTGCCAGTCTTGGGGCA230.14619883040935672No Hit
CTTCAAATCAGGCCCTCCCCTAAACTCTCCTTTATACCATGCTCCAGATA230.14619883040935672No Hit
AATATACCCTTTGACACAGAAATTCTGCTTCTCAGAGCTTACCCTCAGGA230.14619883040935672No Hit
CTATTAGATATCTCGACTATTTAGACATTGAACAGATCATCTTTGCTAAC230.14619883040935672No Hit
TCCCAGGACCCTGAAATGACCTGAGCTGAAGGCAGACGCTTAACGATTGA230.14619883040935672No Hit
ATTTACAGCCCTGTGAAAGCCGTCAGTAACTATTATTGTGGTTAAGGTGT220.1398423595219934No Hit
AACGAGGGGCGCCTGGATGGCTGTCAGTCAGTCAGGTGTCTGCCTTTGGC220.1398423595219934No Hit
AGTTAACACAGATTTTTCAGTTTATCAAAATGTTTGCCAAACAAAACAAA220.1398423595219934No Hit
GTCTTGGGGCATCTGGGTGGCTCATTCGGTTAAAAGCATCTGCCTTCAGC220.1398423595219934No Hit
ATTTACATGAACTCTGACATCTTGCCAAACTCACATTTTTTACACATCAA220.1398423595219934No Hit
GTACTAGGGCCAGGGTCTCTCTAATGAGAGACCACAGGACACCTTAACCA220.1398423595219934No Hit
GTGGCAGGGAGAGGGAGAACGAGGCTCGTGCTGAGCAGGGAGCATGATGT220.1398423595219934No Hit
AGTAGAAACAAGGCAAAGACTTAGTAAAGGACAGTAGTTAAATTTCCATG210.13348588863463007No Hit
GTCCAAGGTAGGATTTAAGAAAATTAAGCTACCAAAAGAAAAACATCAAC210.13348588863463007No Hit
CTTAATAACTATCAGATAAATTGACTATTAGATATCTCGACTATTTAGAC210.13348588863463007No Hit
CCCCTAAACTCTCCTTTATACCATGCTCCAGATAGGGTGCTGAGAGAGAG210.13348588863463007No Hit
GAGTGGGAGAGGGAGATGCAGGCTGTCGAGCTGAGCAGGGAGCCTGATGC210.13348588863463007No Hit
GATCATCTTTGCTAACCGTTGGTATTTGGTGGGTGTGTCTGCTAAGTTGG210.13348588863463007No Hit
GTGTATGATGAAACAAGTTTGGAATGAGCTAAACTATCAATAAGAGGCAG210.13348588863463007No Hit
CTTCAAGACTAATGAGGGACAGAAAATGTAGACAGGAAAGGGGGCAGGAC200.12712941774726672No Hit
TCCCAAGACTCCGTCTGGTTACATTACCAAACTCCCCATAGCTTATAAGG200.12712941774726672No Hit
CCCTTTGACACAGAAATTCTGCTTCTCAGAGCTTACCCTCAGGAAATGAG200.12712941774726672No Hit
GTATTACTTCAGACTGGCAACCATTTGCTCAAATCTTGAACCCAAATATC200.12712941774726672No Hit
TAATTATATTGGAAACTCCATTGATGTATTGGGGAAAAAAAATCACTGTG200.12712941774726672No Hit
CAATAATAGTTACTGACGGCTTTCACAGGGCTGTAAATTGGAGATGGGAG200.12712941774726672No Hit
GTCTACAGAAACTGGGCTGAAATTGCCTAGATTGAGTATCCTGCATCCCT200.12712941774726672No Hit
GTACCAGGCTATTTATAAGAACCTTGTGTATGATGAAACAAGTTTGGAAT200.12712941774726672No Hit
AACTAGAAGAGTGTGATGTTAGAAGTCTAGGAAGAAGTAATTCAGAGAGG200.12712941774726672No Hit
GGCAGGGAGAGGGAGAACGAGGCTCGTGCTGAGCAGGGAGCATGATGTGG200.12712941774726672No Hit
GTTTAGGGGAGGGCCTGATTTGAAGAGAGGAGGGAGGAGATGGCAGGATA200.12712941774726672No Hit
CTATTATTGTGGTTAAGGTGTCCTGTGGTCTCTCATTAGAGAGACCCTGG190.12077294685990338No Hit
ATAGTGACCCAGTGAAGTACTGCATCTTTGGATGAAGATCTACAACAGTC190.12077294685990338No Hit
TGTGTACCTTGTGTCATTTAAGTGCTGCATAGTATACTGCTGTATGGTTG190.12077294685990338No Hit
ATTCAAGCTTCTGGGAAGTACTTTGGATTGTTGGAATTACAGACTTCATT190.12077294685990338No Hit
GTATAAAGGAGAGTTTAGGGGAGGGCCTGATTTGAAGAGAGGAGGGAGGA190.12077294685990338No Hit
TCCTGCATCCCTGCAAATTTGAAGTCCTGGTTTTCTAGGCAGAAACCATC190.12077294685990338No Hit
GATCAAGGTTGTACCTGCAGGATGAGATGCAGGGGATTTATTTTACAATA180.11441647597254005No Hit
GTTCTACATGATCTCACCAACTTAACATGTCCAAAGCAAAATCAGTTCCT180.11441647597254005No Hit
GTTGTACCACAGTCTAGCCTGCCTCTTATTGATAGTTTAGCTCATTCCAA180.11441647597254005No Hit
GTATACTGCTGTATGGTTGTACCACAGTCTAGCCTGCCTCTTATTGATAG180.11441647597254005No Hit
GTACTGTAGCCATGTGCATGTTCCACTCATTTCCTGAGGGTAAGCTCTGA180.11441647597254005No Hit
GCTTTAGCCAAGATCACCAATTCCTTCTGTCTTGCTAAACTCAAAGGCCA180.11441647597254005No Hit
GTCTTGAAGAGTTCCATAGTTTTTAAATATCAGCCACATGCTGGATACCA180.11441647597254005No Hit
TTTCTGTAGACTGTAGTTTTGAGAACAGAGTTGCTAAGGGTCTCAGAGGG180.11441647597254005No Hit
ATTTATTTCCCTAGAGAGGGAACACGAGCAGGGTGAGTGGGAGAGGGAGA180.11441647597254005No Hit
TTGTAGATCTTCATCCAAAGATGCAGTACTTCACTGGGTCACTATTGCAC180.11441647597254005No Hit
ACTTCAGACTGGCAACCATTTGCTCAAATCTTGAACCCAAATATCAATCT180.11441647597254005No Hit
CTACAGAAACTGGGCTGAAATTGCCTAGATTGAGTATCCTGCATCCCTGC180.11441647597254005No Hit
ACATTGAACAGATCATCTTTGCTAACCGTTGGTATTTGGTGGGTGTGTCT180.11441647597254005No Hit
GGGAAAGGATGCCTATTTATTTCATCATCCAGTTAACACAGATTTTTCAG170.10806000508517671No Hit
ATCTACAACAGTCATTCACAGGAAATGAGTAATACTGCTCCCATCTCCAA170.10806000508517671No Hit
TGGCAAAGGGATCTGACAGCCAACTTAGCAGACACACCCACCAAATACCA170.10806000508517671No Hit
GCTTATAAGGTAACGAAACCAAACTGGTATGGGAACAAAAGTTCGGTAAT170.10806000508517671No Hit
GTGAAGTACTGCATCTTTGGATGAAGATCTACAACAGTCATTCACAGGAA170.10806000508517671No Hit
GTCTTATAATGAAAATGAGGTTGTTTTGCACACTGTTGGGAAGTCTGCCT170.10806000508517671No Hit
GTCTAGCCTGCCTCTTATTGATAGTTTAGCTCATTCCAAACTTGTTTCAT170.10806000508517671No Hit
CTCCCTGGGTTCAGTCTCTCTCTCAGCACCCTATCTGGAGCATGGTATAA170.10806000508517671No Hit
CGAGCAGGGTGAGTGGGAGAGGGAGATGCAGGCTGTCGAGCTGAGCAGGG170.10806000508517671No Hit
GTTTTTAAGAATCCACAATGAGGGTTGCTGGGTAGCTCAATCGTTAAGCG170.10806000508517671No Hit
TCTTTAAAATGGGAATAACGAGGGGCGCCTGGATGGCTGTCAGTCAGTCA170.10806000508517671No Hit
CCCCAAGACTGGCAAATATTTTTTGTTTTGTTTGGCAAACATTTTGATAA170.10806000508517671No Hit
CAGCAAAATAAGGCATTTTAAGCCCATGAAAAGGAATTTCAGAATGAAGT170.10806000508517671No Hit
GTACATATTATTTTTCTCCTTTATCTACTTTTTAAGGCACAATTTACCTG170.10806000508517671No Hit
ATGTTAACTTAACCTTCTGAATGCTTCCCCATCTTTAAAATGGGAATAAC170.10806000508517671No Hit
GTTTCTGTAGACTGTAGTTTTGAGAACAGAGTTGCTAAGGGTCTCAGAGG170.10806000508517671No Hit
CTCCTGAGCAGAGAGCCCAATGCAGCCAAAGGCAGACACCTGACTGACTG170.10806000508517671No Hit
CACTAGATATCTGCTATTGTCTTCTCAACCAATCCCAATCAAGGGTCCAT170.10806000508517671No Hit
AGCAGGGTGAGTGGGAGAGGGAGATGCAGGCTGTCGAGCTGAGCAGGGAG170.10806000508517671No Hit
CACTTAATGTTAACTTAACCTTCTGAATGCTTCCCCATCTTTAAAATGGG160.10170353419781336No Hit
CATTAGAGAGACCCTGGCCCTAGTACTGTAAAAGCTTCTACCGACACTAC160.10170353419781336No Hit
GTTAAAGCCATCCACTTCAGATATTTCGTATCTTAATAACTATCAGATAA160.10170353419781336No Hit
ATACTGCTCCCATCTCCAATTTACAGCCCTGTGAAAGCCGTCAGTAACTA160.10170353419781336No Hit
GCTGTAAATTGGAGATGGGAGCAGTATTACTCATTTCCTGTGAATGACTG160.10170353419781336No Hit
ATCTATGATTACTAATTTACTCTCCCACCAGCAATGGGAAAGGATGCCTA160.10170353419781336No Hit
GGTATTTGGTGGGTGTGTCTGCTAAGTTGGCTGTCAGATCCCTTTGCCAT160.10170353419781336No Hit
GCTCTATCCTGCCATCTCCTCCCTCCTCTCTTCAAATCAGGCCCTCCCCT160.10170353419781336No Hit
TCACAGGGCTGTAAATTGGAGATGGGAGCAGTATTACTCATTTCCTGTGA160.10170353419781336No Hit
GTGTAAAGCAAAGGGGCTCAAGTTTATATAACAGCCCACAAATATTTTTC160.10170353419781336No Hit
CTTCTCAACCAATCCCAATCAAGGGTCCATTCCCTCCTTTCCACTGAATT160.10170353419781336No Hit
GTCTCAGAGGGGTGACTTTTGACAAGTTCATTCCTGCCTGTCACGGCCCA160.10170353419781336No Hit
ATCGTTAAGCGTCTGCCTTCAGCTCAGGTCATTTCAGGGTCCTGGGATAG160.10170353419781336No Hit
TTGCTGGGTAGCTCAATCGTTAAGCGTCTGCCTTCAGCTCAGGTCATTTC160.10170353419781336No Hit
CCTTTACTCCTCCTCCTCTAATTATAGGGATGTGCAGTCCTGCCCCCTTT160.10170353419781336No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCCCT259.2143635E-487.06
TTCCCTA259.2143635E-487.07
ATTTCCC259.2143635E-487.05
TATTTCC300.001900323272.54
TCCCTAG300.001900323272.58
CGAGGCT400.00594098154.3758
ACGAGGC400.00594098154.3757
GAGGCTC400.00594098154.3759
TTGAAAA1600.00435080248.15625100-104
AAAAAAA7909.276846E-115.689873105-109
Produced by FastQC (version 0.11.2)