FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2435_day_14_nw.35100000088659.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2435_day_14_nw.35100000088659.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107893
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA3970.36795714272473656No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG3190.29566329604330216No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA1620.15014875849220988No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT1530.14180716079819822No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1250.075.41
TCGAATG300.001931046572.59
GAGCAAA2100.069.0476151
AGCGAAA1500.062.8333362
CGAAAGC1550.060.8064544
TATAGAC400.00603631654.3752
CAGCTCG1258.731149E-1152.2000059
GCGAAAG1850.050.945953
AGCAAAA2900.050.0000042
GGAGCGA604.9253507E-448.3333321
GCAAAAG3350.043.2835853
CAAAAGC3700.039.189194
GTTGATG750.00147751938.6666681
CCCCTGA2109.094947E-1237.97619145
TTTGCGG1157.0836777E-637.826088145
AAAGCAG6600.037.3484886
TATACAG2052.8558134E-1035.3658525
GCAGGGT1251.254675E-534.8000039
GTATTAA1102.4218383E-432.9545481
ATACAGC2051.1186785E-831.8292686
Produced by FastQC (version 0.11.2)