FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2435_day_14_ll-e.3510000008883d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2435_day_14_ll-e.3510000008883d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163921
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA10730.6545836103976915No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC7530.4593676222082589No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA4290.26171143416645826No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA3820.23303908590113528No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA2700.16471349003483385No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA2510.1531225407360863No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA2400.14641199114207454No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT2230.1360411417695109No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC2210.13482104184332697No Hit
CTTAACCACTCAGACACCCAGGCATCCCAACATTAAACAGATTTTTACCT2100.1281104922493152No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA2020.1232300925445794No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC1930.11773964287675161No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT1920.11712959291365964No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1800.1098089933565559No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC1760.10736879350418799No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1690.10309844376254415No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA1690.10309844376254415No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA1690.10309844376254415No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1680.10248839379945218No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG1650.10065824391017623No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAC100.0070854914145.000023
GAGCGAA1400.098.392861
AGCAAAA9350.094.598932
CAAAAGC9450.093.597894
GCAAAAG9500.093.105263
GAGCAAA10650.083.732391
AAAAGCA11550.077.2077945
GCGAAAG1700.076.764713
AAAGCAG13350.076.573036
TGATAGC300.00193284672.51
CGAAAGC1850.070.5405354
AGCGAAA1850.070.5405352
AAGCAGG18750.054.133337
GCAGGGG3850.050.844169
GCAGGAG4300.045.523269
AGCAGGA5600.042.7232138
ACCGTGC1557.3123374E-1042.0967758
GCATTAA1053.8045273E-641.428571
GTATAAG700.001054054841.428571
CCGTGCT1609.986252E-1040.7812549
Produced by FastQC (version 0.11.2)