FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2434_day_14_ul-b.351000000888f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2434_day_14_ul-b.351000000888f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93268
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA8260.8856199339537676No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC5960.639018741690612No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA3350.3591799974267702No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA3230.3463138482652142No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC2190.23480722219839603No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA2140.22944632671441437No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA2090.22408543123043273No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1980.2122914611656731No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1810.19406441652013554No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1550.16618776000343097No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA1510.16189904361624566No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1500.16082686451944933No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1440.15439378993867137No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1440.15439378993867137No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA1410.15117725264828236No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC1390.1490328944546897No Hit
CTTAACCACTCAGACACCCAGGCATCCCAACATTAAACAGATTTTTACCT1380.14796071535789337No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT1340.14367199897070806No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG1330.14259981987391174No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1310.14045546168031908No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG1310.14045546168031908No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA1260.13509456619633745No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC1260.13509456619633745No Hit
GGTTAATCTATTTTTATTAAATATATTTATTGTCATGGTTGGGCTTGAGT1250.1340223870995411No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA1220.1308058498091521No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT1170.12544495432517047No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC1150.12330059613157782No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC1150.12330059613157782No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1140.1222284170347815No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG1110.11901187974439251No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT1100.11793970064759617No Hit
CTGATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCA1090.11686752155079985No Hit
GTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATT1030.11043444697002187No Hit
GTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCGGG1010.1082900887764292No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG1000.10721790967963289No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG980.10507355148604022No Hit
TCCCAGGACCCTGGGATCATGACCTGAGGTGAAGGCAGACAATCGACTGA980.10507355148604022No Hit
TCCCAGGACTTTGGAATCATGACCTGAGCTGAAGGCAGACACTTAACAAC960.10292919329244757No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT950.10185701419565124No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT940.10078483509885491No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAAGT100.007080628145.08
GAGCGAA1300.0111.538471
AGCAAAA6150.0103.739832
GCAAAAG6400.099.68753
CAAAAGC6900.092.463774
AAAAGCA7250.087.05
TCCTACA259.359885E-487.02
AAAGCAG9350.082.967926
GAGCAAA8100.078.7654341
CGAAAGC2000.072.54
CTCCATA300.001930223472.52
GGCATTA406.631578E-572.59
CATCGGC406.631578E-572.55
ATCGGCA406.631578E-572.56
GCGAAAG2100.069.047623
AGCGAAA2100.069.047622
GCAGGAG2300.066.1956569
ACCATCG451.1882113E-464.444443
AAGCAGG12850.060.369657
TCGGCAT502.0007629E-458.07
Produced by FastQC (version 0.11.2)