FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2433_day_14_nw.351000000885c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2433_day_14_nw.351000000885c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101255
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA2260.2231988543775616No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG1720.16986815465902919No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGGC100.0070815203145.0145
CGTAATT100.0070815203145.02
CGAAAGC1300.0100.384623
GCGAAAG1400.093.214292
AGCGAAA1550.084.193541
GAGCGAA554.3544787E-879.090911
GAGCAAA1800.076.527791
GCAAAAG2350.055.5319183
TTGGCGG400.00603525354.3758
CAAAAGC2400.054.3754
CTTGGCG400.00603525354.3757
AGCAAAA2600.050.192312
TTGTATA450.00961432448.3333362
AAGTGTG657.305265E-444.6153877
GAAAGCA3150.041.4285744
ATAGTAC700.001052143841.428573
GTAGGAC750.001477091138.6666683
ATCTTAC750.001477091138.6666681
TGATATA850.00273041734.1176452
TAATACA1601.8551746E-631.718754
Produced by FastQC (version 0.11.2)