FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2432_day_14_nw.351000000886a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2432_day_14_nw.351000000886a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98785
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA3650.3694892949334413No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG2410.24396416459988865No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA2290.23181657134180292No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1370.13868502302981223No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT1360.1376727235916384No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT1040.10527914157007644No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA3450.088.260871
GAGCGAA900.080.555561
AGCGAAA1000.072.52
AGCAAAA4450.070.0561752
ATTATAC350.003556994562.1428573
GCAAAAG5050.061.7326773
CGAAAGC1200.060.4166684
CCCACGG400.00603481354.375145
GCGAAAG1353.6379788E-1253.70373
CAAAAGC5750.052.956524
AAAAGCA6100.049.9180345
TAATATC604.923316E-448.3333324
GTATAAT450.00961362748.3333321
TACACCC450.00961362748.3333325
AAAGCAG8150.047.1472366
TATTATA657.3043845E-444.6153872
TATTAAG907.433488E-540.277782
ATTAAGG750.00147691438.6666683
GTCTATA750.00147691438.6666682
AAGCAGG9550.036.4397937