FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2431_day_8_nw.351000000886f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2431_day_8_nw.351000000886f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57421
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA2230.3883596593580746No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA1980.344821580954703No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT1560.2716776092370387No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG1010.17589383674962122No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC760.13235575834624963No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA760.13235575834624963No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG710.1236481426655753No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA710.1236481426655753No Hit
GAGCAAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCATAGGA600.10449138816809181No Hit
GCACTGCAGGGGGGGGTGCTCTGGGGGAATGAAAGAGGGAGGCCCCCGCA590.10274986503195695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTA100.007073584144.999985
GTAGACC100.007073584144.999988
GAGCAAA2300.0113.478261
GAGCGAA850.0110.8823551
AGCAAAA3200.081.562492
CAAAAGC3450.075.6521764
GCAAAAG3500.074.571433
AGCGAAA1200.072.52
AAAAGCA3900.066.923085
AAAGCAG5900.060.2118646
GCGAAAG1500.058.03
CGAAAGC1600.054.3749964
ATTTACA450.00959326748.3333322
ATAATAG450.00959326748.3333323
TATAATA450.00959326748.3333322
GCACTAC450.00959326748.333332145
CACTTTG450.00959326748.3333327
GCAGGTA700.001048331641.428579
AAGCAGG8350.039.9401177
TAAGAGA800.002020692236.2499964
Produced by FastQC (version 0.11.2)