FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2431_day_8_ll-e.351000000887c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2431_day_8_ll-e.351000000887c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72526
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA6660.9182913713702673No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC4350.5997849047238232No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT2400.330915809502799No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA2370.32677936188401396No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA2180.30058186029837575No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA1930.2661114634751675No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1740.2399139618895293No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA1600.22061053966853267No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1470.2026859333204644No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC1330.18338251109946777No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1210.16683672062432783No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA1200.1654579047513995No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1080.14891211427625956No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1050.14477566665747457No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1040.14339685078454623No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1000.1378815872928329No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA960.1323663238011196No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC960.1323663238011196No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA940.12960869205526296No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT940.12960869205526296No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG930.1282298761823346No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT920.12685106030940627No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC920.12685106030940627No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA910.12547224443647795No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC900.12409342856354963No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA880.12133579681769296No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA880.12133579681769296No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC870.11995698094476465No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA840.11582053332597965No Hit
GAGCAAAAGCAGGGGATTAGGAGTACACTTTCTGTGAGAAGCTCTGAGTA780.10754763808840967No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT770.10616882221548135No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG770.10616882221548135No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC750.10341119046962469No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG730.10065355872376804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1150.0132.391311
AGCGAAA1650.092.272732
AGCAAAA4850.088.195882
GCGAAAG1750.087.03
GCAGGAT259.351417E-487.09
GCAAAAG4950.086.414143
CGAAAGC1850.082.2972954
AAAGCAG7250.082.06
GAGCAAA5450.081.146791
CAAAAGC5450.078.486244
AGCAGGA2050.077.804888
AAAAGCA5950.073.1092455
AACTGGT300.001928483472.54
GAGTTAG300.001928483472.51
GAAAGCA2300.066.1956565
AAGCAGG9650.062.357517
GCAGGTT350.003553178962.1428579
GCAGGAG1800.060.4166689
GCAGGGG2750.057.9999969
AGCAGGT805.7977013E-754.3758
Produced by FastQC (version 0.11.2)