FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2430_day_8_ul-b.351000000887de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2430_day_8_ul-b.351000000887de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64982
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC2050.3154719768551291No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1630.2508386937921271No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC1560.24006647994829336No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA1560.24006647994829336No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA1530.23544981687236466No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT1380.21236650149272107No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA1350.20774983841679232No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1320.20313317534086361No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT1160.1785109722692438No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1120.17235542150133884No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA1090.1677387584254101No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT1030.15850543227355268No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG1020.15696654458157644No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT970.14927210612169522No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA870.13388322920193285No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG870.13388322920193285No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA820.12618879074205164No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT810.12464990305007541No Hit
GAGCAAAAGCAGGGACTTTTGGGCAGCATTAAGATGTATGAGGTGTATTC800.12311101535809915No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT790.12157212766612292No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC780.12003323997414668No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA750.11541657689821798No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA740.11387768920624172No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC730.11233880151426548No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG690.10618325074636054No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC680.10464436305438428No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA680.10464436305438428No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG660.10156658767043181No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA650.10002769997845558No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA458.367351E-11112.777781
AGCGAAA950.083.947371
GCAAAAG3400.076.76473
AGCAAAA3550.073.5211262
TTAGCAC300.001927574772.53
AAAAGCA3700.070.5405355
GCGAAAG1150.069.3478242
CGAAAGC1200.066.4583363
CAAAAGC4000.065.254
ATCCTAT350.003551511362.1428571
ATTAGCA350.003551511362.1428572
TTGGAGT350.003551511362.1428574
TAGCACA350.003551511362.1428574
GATTAGC350.003551511362.1428571
GCAGGAT851.2800228E-859.705889
AAAGCAG5250.059.3809556
GCTAAAC1007.021299E-1058.01
AACACTC651.0822381E-555.769234145
AAGGACC1051.0822987E-955.2380948
GAGCAAA4750.054.947371
Produced by FastQC (version 0.11.2)