FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2430_day_8_ll-e.35100000088a8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2430_day_8_ll-e.35100000088a8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133278
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC5030.3774066237488558No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA4890.3669022644397425No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA4470.3353891865124027No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA4370.32788607272017883No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA4290.32188358168639986No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT3230.24235057548882785No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA3030.22734434790438032No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC2750.20633562928615376No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT2620.19658158135626283No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA2590.19433064721859572No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA2360.17707348549648105No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG2290.1718213058419244No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC2230.16731943756659015No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT2150.16131694653281112No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT2110.1583157010159216No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA2040.15306352136136495No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC1890.1418088506730293No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA1770.13280511412236076No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT1760.13205480274313838No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA1710.12830324584702651No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG1690.12680262308858176No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC1680.12605231170935938No Hit
TTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCTTCTGCCAG1650.12380137757169227No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC1650.12380137757169227No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1560.11704857515869085No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA1550.11629826377946847No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG1530.11479764102102373No Hit
GCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACT1510.11329701826257897No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT1510.11329701826257897No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA1490.11179639550413423No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG1480.11104608412491183No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1480.11104608412491183No Hit
AGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCAC1460.10954546136646708No Hit
GAGCAAAAGCAGGGGATTAGGAGTACACTTTCTGTGAGAAGCTCTGAGTA1450.1087951499872447No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA1450.1087951499872447No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG1440.10804483860802233No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA1440.10804483860802233No Hit
AGACTAAACACTGCAGTTAAAGTCAGGGATTTGGGGGCACCTGGGTGGCT1400.10504359309113283No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT1380.10354297033268807No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATCG100.0070840167144.999983
CCATCGG100.0070840167144.999984
ATCGGCA100.0070840167144.999986
GAGCGAA1300.094.807691
AGCAAAA5700.086.491232
AGCGAAA2700.085.925931
GCGAAAG2950.078.6440662
GCAAAAG6400.077.031243
CGAAAGC3150.073.650793
AAAAGCA7550.066.258285
CAAAAGC8550.059.3567284
AAAGCAG10800.059.0740746
GAAAGCA3950.058.7341774
GTAGAAA1550.056.129031
ATCACGA400.00603942954.3749961
CATAAGC400.00603942954.3749969
CAAGGGG553.2088702E-452.7272728
GAGCAAA9950.051.0050281
GCAGGGG4300.050.5813949
ACGACCT450.009620951548.3333324
Produced by FastQC (version 0.11.2)