FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2429_day_8_nw.35100000088580.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2429_day_8_nw.35100000088580.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80946
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2000.2470782991129889No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA1860.22978281817507967No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA1810.22360586069725494No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1460.1803671583524819No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA1250.15442393694561807No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC1200.14824697946779333No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG1190.1470115879722284No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC1040.12848071553875423No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA1030.12724532404318928No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT990.12230375806092952No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT840.10377288562745533No Hit
GCACTGCAGGGGGGGGTGCTCTGGGGGAATGAAAGAGGGAGGCCCCCGCA840.10377288562745533No Hit
GAGCAAAAGCAGGCAAGTGGGAGTTCAGAGAGGAGAACAGTTGCTGAGAG830.10253749413189041No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA3400.0104.485291
GCACTAG301.5905456E-596.6666641
AGCGAAA608.1672624E-1084.5833362
AGCAAAA4350.081.666672
GCAAAAG4550.078.076933
GAGCGAA755.4569682E-1177.3333361
GTACTAT950.076.315781
CAAAAGC5100.069.656864
GCGAAAG754.780304E-967.666673
AAAAGCA5350.066.401875
TATGTTC1009.094947E-1265.255
CTATGTT902.764864E-1064.444444
AAAGCAG6450.062.945746
GTAGATG350.00355466962.1428577
ATAGGTT501.9991696E-458.05
CGAAAGC902.0181687E-856.388894
TAGATGG400.00603088254.3758
TATAGGT553.20132E-452.7272764
ACTATGT1152.4356268E-950.4347843
ATGTTCT1152.4356268E-950.4347846
Produced by FastQC (version 0.11.2)