FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2429_day_8_ll-e.3510000008878e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2429_day_8_ll-e.3510000008878e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100199
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA8100.8083913013103924No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC5250.5239573249234024No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA3070.30639028333616103No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT2570.2564895857244084No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2470.2465094462020579No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA2440.24351540434535274No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA2150.21457299973053623No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA2050.20459286020818573No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA2010.20060080439924552No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC1630.1626762742143135No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1610.16068024630984343No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC1470.1467080509785527No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC1450.1447120230740826No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1440.1437140091218475No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1360.1357298975039671No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1360.1357298975039671No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1350.13473188355173207No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1340.13373386959949699No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA1320.1317378416950269No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA1160.11576961845926606No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA1140.11377359055479595No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC1120.11177756265032585No Hit
GAGCAAAAGCAGGGGATTAGGAGTACACTTTCTGTGAGAAGCTCTGAGTA1100.10978153474585574No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT1100.10978153474585574No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1080.10778550684138563No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA1080.10778550684138563No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC1080.10778550684138563No Hit
GAGCAAAAGCAGGCAAGGGCCCCGAGCCATGGAAAACAGTCGACAGCCCA1070.1067874928891506No Hit
GATACCACCTTCTGCCATGACCTTGGTCACAGACAGTACAGACAGACAGA1040.10379345103244543No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT1030.10279543708021038No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG1030.10279543708021038No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC1020.10179742312797532No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG1020.10179742312797532No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCAC100.0070814057145.06
CTAGACC100.0070814057145.03
GTATAGC151.2267285E-4145.01
AGCAAAA6900.0104.021742
GCAAAAG7550.094.105963
CAAAAGC7600.093.486854
AAAGCAG9650.081.891196
GAGCGAA1100.079.090911
GAGCAAA9350.076.764711
AAAAGCA9300.076.397855
AAGCAGG12600.062.7182547
TAGGAGT350.003557142362.1428577
GCATTAA851.2894816E-859.7058831
GCAGGAT851.2894816E-859.7058839
GCGAAAG1350.059.0740743
GCAGGAG2950.058.9830559
TTAAGTC502.0014825E-458.02
AGCAGGA3900.057.6282048
CGAAAGC1400.056.9642874
AGCGAAA1400.056.9642872
Produced by FastQC (version 0.11.2)