FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2428_day_8_nw.35100000088530.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2428_day_8_nw.35100000088530.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109335
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG4730.43261535647322447No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA3250.29725156628709926No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG3180.29084922485937714No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2350.21493574793067177No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA1550.14176613161384735No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT1460.1335345497782046No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC1370.12530296794256185No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG1280.11707138610691911No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG1200.10975442447523666No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1110.10152284263959391No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1100.1006082224356336No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1150.094.565221
AGCGAAA1100.092.272722
CGAAAGC1150.088.260874
GAGCAAA4350.085.000011
GCGAAAG1250.081.23
AGCAAAA5600.064.732142
CGTTACA702.3151733E-762.1428573
GTTACAC753.7300742E-758.0000044
TTACACC902.0268999E-856.388895
CAAAAGC6550.055.343514
AAAGCAG8500.054.5882346
CCGATTA400.006036533554.3759
ATACTCC400.006036533554.3754
TTATACC400.006036533554.3753
GCCGTTA805.8252044E-754.3751
CCGTTAC805.8252044E-754.3752
GCAAAAG6750.053.70373
AAGCAGG9050.052.872937
ATAAAGT553.2063125E-452.7272723
CTAGTGC701.6859796E-551.7857139
Produced by FastQC (version 0.11.2)