FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2428_day_8_ll-e.3510000008892f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2428_day_8_ll-e.3510000008892f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56619
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2470.43624931560077007No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC2340.41328882530599265No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT1810.31968067256574645No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA1810.31968067256574645No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT1690.29848637383210586No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA1660.29318779914869564No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG1560.27552588353732843No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1510.2666949257316449No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC1430.2525653932425511No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT1370.24196824387573077No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1100.1942810717250393No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1070.18898249704162914No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1050.1854501139193557No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT970.17132058143026194No Hit
AGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCAC930.16425581518571505No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC910.1607234320634416No Hit
ATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGGCATTTTTCTC870.1536586658188947No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA780.13776294176866422No Hit
AGCGAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAATA770.13599675020752752No Hit
GAGCGAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA760.13423055864639077No Hit
GGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAG750.13246436708525408No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA750.13246436708525408No Hit
GAGCAAAAGCAGGGTGTGGGGAGGCATTTTATTAAAAAAAATTAACAAAC730.1289319839629806No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC730.1289319839629806No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA730.1289319839629806No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT730.1289319839629806No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC720.12716579240184392No Hit
TTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCG710.12539960084070717No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA710.12539960084070717No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT700.12363340927957046No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA690.12186721771843374No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG680.12010102615729702No Hit
GCAGAGGGAGAGGAGGAAGCAGGCTCCCCACTCAGCAGGGAGCCCAATGT660.1165686430350236No Hit
AGCGAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCAC640.11303625991275013No Hit
ATCTAGTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCC640.11303625991275013No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG630.11127006835161342No Hit
AGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCC630.11127006835161342No Hit
GTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCT630.11127006835161342No Hit
GCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACT620.10950387679047668No Hit
ATATTATCTTACCCCTCTGCCATAGGCCTTTATTGTTTAGGTAATTAAAA610.10773768522933998No Hit
ATGTAGTATGATTCCACTTATATAAAAACAAACAAAAAAAGGAAAAGGTC610.10773768522933998No Hit
TTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATT610.10773768522933998No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT610.10773768522933998No Hit
GATGTATGTAGTATGATTCCACTTATATAAAAACAAACAAAAAAAGGAAA600.10597149366820326No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA600.10597149366820326No Hit
GTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTC600.10597149366820326No Hit
GAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAAT590.10420530210706655No Hit
GGTTAAGAGTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGT590.10420530210706655No Hit
GAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTA590.10420530210706655No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA590.10420530210706655No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC580.10243911054592982No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG580.10243911054592982No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA580.10243911054592982No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC570.10067291898479308No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT570.10067291898479308No Hit
GGTTAATCTATTTTTATTAAATATATTTATTGTCATGGTTGGGCTTGAGT570.10067291898479308No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGAC100.007073325145.0145
GAGAGTG100.007073325145.07
ATACTCT100.007073325145.06
GAGCGAA600.0132.916671
AGAGTGT259.340732E-487.08
AGCAAAA3300.083.484852
CGAAAGC1200.078.5416643
AAAAGCA3550.077.605645
GCAAAAG3600.076.527783
GCGAAAG1250.075.42
AGCGAAA1250.075.41
GAGCAAA3950.071.582281
GAAAGCA1400.067.321434
AAAGCAG5400.065.787036
GCAGGGG2150.060.6976749
CAAAAGC4650.059.2473144
GCAGGTT400.00602155254.3758
TCTTACC701.6755677E-551.785717
GCAGGAT701.6755677E-551.785719
CTTACCC701.6755677E-551.785718
Produced by FastQC (version 0.11.2)