FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2427_day_5_ll-e.35100000088820.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2427_day_5_ll-e.35100000088820.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131706
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG4870.36976295688882815No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG4370.3317996142924392No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA4330.3287625468847281No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC2600.1974093815012224No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2500.18981671298194464No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA1870.14198290131049457No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA1620.12300123001230012No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA1590.12072342945651678No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG1490.113130760937239No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1370.10401955871410565No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTGCG100.007083923145.09
GCACGGC100.007083923145.02
GAGCGAA800.0135.93751
GCAGGTA850.085.294129
GAGCAAA6950.083.453231
GCGAAAG1850.074.459462
AGCGAAA1850.074.459461
AGCAAAA7800.073.429492
GCAGGGT2200.072.500019
CGAAAGC1900.072.499993
GCAAAAG8150.069.3865053
CAAAAGC8350.068.592824
AAAGCAG10450.064.521536
AAAAGCA9650.059.3523335
AAGCAGG11900.057.2689067
GGATAGC400.006039272554.3755
TATACGA400.006039272554.3752
GCAGGAG1500.053.1666689
ACAAGGT858.865263E-751.176473
GAAAGCA2850.050.8771934
Produced by FastQC (version 0.11.2)