FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2425_day_5_ul-b.35100000088a7e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2425_day_5_ul-b.35100000088a7e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228029
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA9550.4188063798902771No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG8880.38942415219116866No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC8360.3666200351709651No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA7790.34162321459112654No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA5880.25786193861307116No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA5130.22497138521854676No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA4930.21620057098000692No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG4910.21532348955615294No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC4490.1969047796552193No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG4060.17804752904235865No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC4030.17673190690657767No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT3990.1749777440588697No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC3870.16971525551574582No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA3620.158751737717571No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA3540.15524341202215508No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA3540.15524341202215508No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA3280.1438413535120533No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC3160.13857886496892938No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT3080.13507053927351345No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG3070.13463199856158647No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT3060.13419345784965947No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA3040.1333163764258055No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT2950.12936951001846256No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA2930.12849242859460858No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT2860.12542264361111966No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA2830.12410702147533867No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA2800.1227913993395577No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC2780.1219143179157037No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2730.11972161435606875No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA2710.11884453293221477No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC2650.11621328866065282No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA2610.11445912581294485No Hit
GCGTGAACACAAATCCTAAAATTCCCTTAGTCAGAGGTGACAAGATTGGT2560.11226642225330989No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC2530.11095080011752892No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG2440.10700393371018599No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC2430.106565392998259No Hit
AGACTAAACACTGCAGTTAAAGTCAGGGATTTGGGGGCACCTGGGTGGCT2400.10524977086247803No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG2350.10305706730284307No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA2330.10217998587898909No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG2290.1004258230312811No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA2290.1004258230312811No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTG100.007087298145.01
GGTAAAC100.007087298145.03
TCGTGAT100.007087298145.03
GAGCGAA1550.0126.290331
CAAAAGC12350.078.663974
GTAGAAA1950.078.076931
AGCAAAA12850.076.167312
GAGCAAA13250.075.509431
GCAGGTA1350.075.185199
AAAAGCA13400.073.0410465
AGCGAAA3100.070.161291
AAGCAGG17250.067.6666647
AAAGCAG17450.066.891116
GCAGGAG3500.066.285719
CGAAAGC3350.064.925373
GCGAAAG3400.063.9705852
GACAGTA350.003562974362.1428577
GCAAAAG15650.062.076683
AAGGTCG1200.060.416679
CTGACGG902.0401785E-856.38889145
Produced by FastQC (version 0.11.2)