FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2425_day_5_ll-e.35100000088813.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2425_day_5_ll-e.35100000088813.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121667
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG13571.1153394100290137No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA12150.9986274010208191No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT7980.6558886140037973No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG6040.4964369960630245No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC4980.40931394708507646No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA4650.3821907337240172No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC4220.34684836479900055No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT4020.33041005367108583No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA3980.32712239144550287No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA3540.2909581069640905No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG3460.28438278251292465No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT3440.28273895140013316No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA3300.27123213361059284No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTATTCTCTTG3190.26219106249023977No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG3150.2589034002646568No Hit
GTTCATTGATGCTTAATGCTGGGCCATGTCCATTATTCTCTTGTCTGCTG2850.23424593357278473No Hit
GAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC2780.2284925246780146No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2710.22273911578324443No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA2640.2169857068884743No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA2590.2128761291064956No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT2540.20876655132451694No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC2540.20876655132451694No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC2430.19972548020416384No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG2270.18657483130183206No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2250.18493100018904057No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC2100.17260226684310453No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG2100.17260226684310453No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG2080.17095843573031308No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG2040.16767077350473011No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG2020.16602694239193866No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2000.16438311127914718No Hit
ATGCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCAT1970.16191736460995998No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT1920.15780778682798127No Hit
GAGCAAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAA1910.15698587127158556No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG1880.15452012460239833No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG1830.15041054682041968No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG1820.14958863126402394No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG1750.14383522236925378No Hit
GGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGC1740.14301330681285807No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT1710.14054756014367084No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT1660.13643798236169216No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA1640.13479415124890068No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT1600.13150648902331774No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT1600.13150648902331774No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT1570.12904074235413054No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA1550.12739691124133906No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1550.12739691124133906No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT1540.12657499568494332No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG1530.12575308012854758No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT1500.1232873334593604No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG1480.12164350234656891No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT1470.12082158679017317No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT1470.12082158679017317No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA1460.11999967123377743No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA1450.11917775567738172No Hit
GCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT1440.11835584012098598No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA1440.11835584012098598No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA1440.11835584012098598No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC1390.1142462623390073No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC1370.11260243122621581No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG1350.11095860011342434No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA1310.1076709378878414No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT1310.1076709378878414No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC1260.10356136010586273No Hit
GACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAA1240.10191752899307124No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTCA100.0070832623145.01
TCACATA100.0070832623145.05
GCGAAGC100.0070832623145.03
GGTCCGT100.0070832623145.06
CCTACTA202.1230117E-6145.05
GCCGCTC100.0070832623145.08
CACCTAC202.1230117E-6145.03
ATACGTA100.0070832623145.02
GGTTATT100.0070832623145.01
TGGTCCG100.0070832623145.05
TACGTAA100.0070832623145.03
TGCCGCT100.0070832623145.07
GAGCGAA1450.0135.01
GAGCAAA7400.0119.527031
AGCGAAA1800.0100.694442
GCGAAAG1850.097.972983
AGCAAAA9000.097.472232
TCCACCT301.5937901E-596.6666641
CGAAAGC1900.095.394744
CAAAAGC9350.094.598934
Produced by FastQC (version 0.11.2)