FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2423_day_3_ul-b.35100000088a61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2423_day_3_ul-b.35100000088a61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211367
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA12080.5715177865986649No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC10180.481626744004504No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA8710.4120794636816532No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA7180.33969351885582894No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA4150.19634096145566718No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC4060.19208296470120692No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA3520.1665349841744454No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT3510.16606187342394982No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA3450.1632232089209763No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA3080.14571811115263972No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC2960.14004078214669272No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC2810.13294412088925897No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA2770.13105167788727662No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT2700.12773990263380755No Hit
AGACTAAACACTGCAGTTAAAGTCAGGGATTTGGGGGCACCTGGGTGGCT2680.1267936811328164No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA2660.1258474596318252No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG2640.12490123813083405No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA2590.12253568437835614No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2580.12206257362786055No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG2510.11875079837439147No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA2490.11780457687340029No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG2460.11638524462191353No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG2450.11591213387141797No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC2430.11496591237042679No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC2430.11496591237042679No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA2410.11401969086943563No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA2340.11070791561596655No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT2240.1059768081110107No Hit
GTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCGGG2230.10550369736051513No Hit
AGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACC2120.1002994791050637No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA100.0070869336145.09
CCGTATC100.0070869336145.08
GAGCGAA1900.095.394741
AGCAAAA10300.083.058252
CAAAAGC10850.078.1797264
GCAAAAG10850.078.1797263
AAAAGCA12850.066.011675
AAAGCAG15700.065.111476
AGCGAAA3150.064.444441
GAGCAAA13550.064.206641
GCGAAAG3550.057.1830982
CGAAAGC3650.055.616443
GCAGGAG4700.055.5319189
AAGCAGG19950.051.24067
AGTTTAG604.936174E-448.3333328
AGTAGAA1950.044.615381
GAAAGCA4750.042.7368434
ACCAACC855.3271506E-542.647068
GTAGAAA2200.039.5454521
CCCTTAT951.0261411E-438.1578941
Produced by FastQC (version 0.11.2)