000000000-ATLLR_l01n01_2422_day_3_ul-b.35100000088956.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-ATLLR_l01n01_2422_day_3_ul-b.35100000088956.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	58684
Sequences flagged as poor quality	0
Sequence length	151
%GC	41

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA	525	0.8946220434871515	No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC	383	0.652648081248722	No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA	212	0.3612569013700498	No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA	206	0.3510326494444823	No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA	169	0.28798309590348303	No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA	134	0.22834162633767296	No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT	123	0.20959716447413265	No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA	113	0.1925567445981869	No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG	110	0.18744461863540318	No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA	107	0.18233249267261945	No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC	101	0.172108240747052	No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC	100	0.17040419875945745	No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA	99	0.16870015677186287	No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA	95	0.16188398882148455	No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA	92	0.15677186285870084	No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT	89	0.1516597368959171	No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC	89	0.1516597368959171	No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA	87	0.14825165292072798	No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG	84	0.14313952695794424	No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG	82	0.13973144298275508	No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC	82	0.13973144298275508	No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT	82	0.13973144298275508	No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA	79	0.13461931701997137	No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT	76	0.12950719105718764	No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA	76	0.12950719105718764	No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC	75	0.12780314906959306	No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC	75	0.12780314906959306	No Hit
GGTTAATCTATTTTTATTAAATATATTTATTGTCATGGTTGGGCTTGAGT	71	0.12098698111921478	No Hit
CAGTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGA	69	0.11757889714402563	No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA	69	0.11757889714402563	No Hit
AGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACC	68	0.11587485515643105	No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC	65	0.11076272919364734	No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG	64	0.10905868720605275	No Hit
TCCCAGGACTTTGGAATCATGACCTGAGCTGAAGGCAGACACTTAACAAC	64	0.10905868720605275	No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT	64	0.10905868720605275	No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT	63	0.10735464521845818	No Hit
GTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCGGG	62	0.1056506032308636	No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG	62	0.1056506032308636	No Hit
ATATATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAAT	60	0.10224251925567446	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGAA	50	1.8189894E-12	116.0	1
AGCAAAA	270	0.0	75.18519	2
GCAAAAG	275	0.0	73.81818	3
CAAAAGC	290	0.0	72.5	4
GCGAAAG	110	0.0	65.909096	2
AGCGAAA	100	9.094947E-12	65.25	1
AAAGCAG	415	0.0	64.63855	6
CCTTATA	45	1.1845469E-4	64.44444	2
CATTGCC	35	0.0035497914	62.142857	8
CGAAAGC	120	0.0	60.416668	3
AAAAGCA	355	0.0	59.225353	5
AGCAGGA	225	0.0	57.999996	8
GAGCAAA	365	0.0	57.60274	1
CCCTTAT	40	0.006022636	54.375	1
GCAGGAG	175	0.0	53.857143	9
AAGCAGG	515	0.0	52.087376	7
GAAAGCA	150	9.094947E-12	48.333332	4
GCAGGAT	45	0.009594302	48.333332	7
TTATACT	65	7.280022E-4	44.615387	4
GAGGCAG	80	0.0020210505	36.25	5

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content