FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2421_day_3_nw.3510000008855a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2421_day_3_nw.3510000008855a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206829
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG15420.7455434199266061No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA15130.7315221753235764No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA11050.5342577684947469No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG10240.49509498184490575No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT8340.4032316551354017No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT5880.28429282160625446No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT4920.23787766705829455No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT4920.23787766705829455No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT4060.19629742444241377No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT3940.1904955301239188No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3900.18856156535108712No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT3790.18324316222580006No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG3680.177924759100513No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC3660.17695777671409715No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA3470.16777144404314676No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC3460.16728795284993883No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC3450.1668044616567309No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT3180.1537501994401172No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG3160.15278321705370135No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT3000.1450473579623747No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA2950.14262990199633513No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT2910.1406959372235035No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2900.14021244603029556No Hit
GCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCG2840.13731149887104807No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT2830.13682800767784015No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC2770.13392706051859266No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG2750.1329600781321768No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC2730.13199309574576099No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC2660.1286086573933056No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA2560.12377374546122644No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA2550.1232902542680185No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA2550.1232902542680185No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2530.12232327188160268No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC2500.12087279830197892No Hit
GAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA2470.11942232472235519No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC2470.11942232472235519No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2430.11748835994952352No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG2400.11603788636989976No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT2390.11555439517669185No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2360.11410392159706811No Hit
GAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG2320.11216995682423644No Hit
AGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTAT2290.1107194832446127No Hit
ATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGA2160.1044340977329098No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC2120.10250013296007814No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT2110.10201664176687023No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG2110.10201664176687023No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT2090.10104965938045438No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA2080.10056616818724647No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC2070.10008267699403856No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCGG100.007086825145.0145
CTAATAT100.007086825145.02
GAGCGAA2000.0141.3751
TTATAGG352.75395E-7103.571431
GCAGGTA3950.0102.784819
AGCGAAA2850.096.6666642
GCAGGGT5350.092.1495369
CGAAAGC3000.091.8333364
GCGAAAG3000.091.8333363
GAGCAAA11900.087.121851
AAGCAGG16450.081.094227
AGCAGGG7600.076.315798
TTTAAAC1050.075.952383
CAATTCG1050.075.952389
AGCAAAA14350.072.752612
TTTTAAA1100.072.500012
GACAGTA300.001933564472.57
CCTACTA1200.072.55
CACCTAC1200.072.53
TCCACCT1200.072.51
Produced by FastQC (version 0.11.2)