FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2418_day_1_nw.351000000885b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2418_day_1_nw.351000000885b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179353
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA7880.4393570221852994No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG6700.3735649807920693No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG5950.33174800533027043No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT4110.22915702553065742No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG4100.22859946585783342No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT3400.18957028876015455No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT3400.18957028876015455No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA3170.17674641628520293No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA2680.1494259923168277No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA2680.1494259923168277No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2600.14496551493423585No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA2410.13437188115058013No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT2390.13325676180493218No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC2380.1326992021321082No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT2340.13046896344081224No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT2340.13046896344081224No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT2140.11931776998433258No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT2130.1187602103115086No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT2130.1187602103115086No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT2100.11708753129303663No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG2090.11652997162021266No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT2010.11206949423762079No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG2000.1115119345647968No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG2000.1115119345647968No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC1930.1076090168550289No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT1900.10593633783655695No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC1890.10537877816373298No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT1880.104821218490909No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA1850.10314853947243703No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC1850.10314853947243703No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG1800.10036074110831711No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAAT151.2283362E-4145.01
GAGCGAA850.0145.01
GCAGGTA2400.0108.759
AGTAGAA850.0102.352941
GCTATAC301.5958654E-596.6666643
CCTACTA301.5958654E-596.6666645
CACCTAC301.5958654E-596.6666643
AGCGAAA1350.091.2962952
GCAGGGT2350.083.2978749
CCACCTA353.4295743E-582.857142
CGAAAGC1650.074.696974
GCGAAAG1650.074.696973
GACAGTA607.9940946E-872.57
ACTATAT406.6483015E-572.499993
CCACTAT406.6483015E-572.499991
TCCACCT406.6483015E-572.499991
AAGCAGG9500.069.447377
GCAAAAG7550.069.139073
CATACTC754.82396E-967.666673
GTAGAAA1300.066.923072
Produced by FastQC (version 0.11.2)