FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2187_day_3_ul-b.351000000888b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2187_day_3_ul-b.351000000888b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159007
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT18811.182966787625702No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA17401.0942914462885283No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG15400.9685108202783526No Hit
GCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTG9020.5672706233058922No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT8920.5609815920053834No Hit
CTCAACAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCT8240.5182161791619236No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA7190.4521813505065815No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC6400.4024980032325621No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT6150.38677542498129014No Hit
ATGCTGGGCCATATCTCTTGTTGAGTGCGGGGTTCTTCTCTTGCCTTCCT5880.3697950404699164No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA5840.3672794279497129No Hit
GCTGGGCCATATCTCTTGTTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGA5620.3534435590885936No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG5310.33394756205701637No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG5160.3245140151062532No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA5050.31759608067569356No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA4560.2867798273032005No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC4370.27483066783223375No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA4330.27231505531203026No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT4240.2666549271415724No Hit
GAGCAAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAA4170.26225260523121624No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT4140.2603658958410636No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA4040.25407686454055484No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC3580.2251473205582144No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG3560.22388951429811269No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG3480.21885828925770562No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT3420.21508487047740038No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC3390.21319816108724773No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG3330.20942474230694247No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC3320.2087958391768916No Hit
GCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCT3230.20313571100643366No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG3220.2025068078763828No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC3190.20062009848623016No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG3020.18992874527536524No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC3020.18992874527536524No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG2900.1823819077147547No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT2860.1798662951945512No Hit
CTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTCCA2830.17797958580439854No Hit
CCATGATGCCGTTGCAACTACACATTCCTGGATTCCCAAGAGGAATCGTT2750.1729483607639915No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2710.170432748243788No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT2660.16728823259353362No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTC2620.1647726200733301No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA2540.1597413950329231No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT2500.15722578251271957No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT2470.15533907312256695No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG2400.15093675121221078No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT2390.1503078480821599No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG2370.14905004182205814No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG2330.14653442930185465No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG2270.14276101052154935No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT2160.1358430760909897No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA2130.13395636670083705No Hit
CAGAATGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAA2100.13206965731068443No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA2090.13144075418063356No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG2050.12892514166043004No Hit
CAACAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTG2030.1276673354003283No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG1960.12326501348997214No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT1940.12200720722987039No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA1940.12200720722987039No Hit
GGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGAGATTAC1910.12012049783971775No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT1880.11823378844956511No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC1870.11760488531951424No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT1860.11697598218946335No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT1820.11446036966925985No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG1790.1125736602791072No Hit
CTACCCCTGTTGTGTTACATATACTGTTCACCACTGTCCAGGCCATGATT1750.1100580477589037No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT1700.1069135321086493No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG1690.10628462897859842No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG1670.10502682271849666No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTC1650.10376901645839491No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC1610.1012534039381914No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTT1600.10062450080814052No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCCC100.007085295145.02
GAGCGAA1750.0140.857131
GAGCAAA9200.0126.8751
CAAGTAC203.8600143E-4108.755
AGCAAAA12550.094.1633452
GCAGGGT3850.092.272739
CAAGGTA406.093687E-790.6254
AGTAGAA1700.089.558831
AGTACAG259.3721325E-487.07
AAGCAGG16450.086.823717
CAAAAGC13900.086.061154
AGCAGGC4400.085.6818168
AAAAGCA14250.083.9473655
GCAGGCA4550.082.857159
GGTAGTT353.4283752E-582.857147
AAAGCAG18150.079.490366
GACAGTA554.3706677E-879.0909047
AGCGAAA3150.075.9523852
CGAAAGC3300.072.54
AGCAGGT7700.071.558448
Produced by FastQC (version 0.11.2)